Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST [Applied Biological Sciences]
Streptomycetes serve as major producers of various pharmacologically and industrially important natural products. Although CRISPR-Cas9 systems have been developed for more robust genetic manipulations, concerns of genome instability caused by the DNA double-strand breaks (DSBs) and the toxicity of Cas9 remain. To overcome these limitations, here we report development of...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Yaoȷun Tong, Christopher M. Whitford, Helene L. Robertsen, Kai Blin, Tue S. Jorgensen, Andreas K. Klitgaard, Tetiana Gren, Xinglin Jiang, Tilmann Weber, Sang Yup Lee Tags: Biological Sciences Source Type: research