Cancers, Vol. 11, Pages 1408: Methylation Assessment for the Prediction of Malignancy in Mediastinal Adenopathies Obtained by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration in Patients with Lung Cancer
Cancers, Vol. 11, Pages 1408: Methylation Assessment for the Prediction of Malignancy in Mediastinal Adenopathies Obtained by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration in Patients with Lung Cancer
Cancers doi: 10.3390/cancers11101408
Authors:
Virginia Leiro
Loretta De Chiara
Mar Rodríguez-Girondo
Maribel Botana-Rial
Diana Valverde
Manuel Núñez-Delgado
Alberto Fernández-Villar
The evaluation of mediastinal lymph nodes is critical for the correct staging of patients with lung cancer (LC). Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a minimally invasive technique for mediastinal staging, though unfortunately lymph node micrometastasis is often missed by cytological analysis. The aim of this study was to evaluate the predictive capacity of methylation biomarkers and provide a classification rule for predicting malignancy in false negative EBUS-TBNA samples. The study included 112 patients with a new or suspected diagnosis of LC that were referred to EBUS-TBNA. Methylation of p16/INK4a, MGMT, SHOX2, E-cadherin, DLEC1, and RASSF1A was quantified by nested methylation-specific qPCR in 218 EBUS-TBNA lymph node samples. Cross-validated linear regression models were evaluated to predict malignancy. According to EBUS-TBNA and final diagnosis, 90 samples were true positives for malignancy, 110 were true negatives, and 18 were false negatives. MGMT, SHOX2, and E-cadherin were the methylation markers that be...
Source: Cancers - Category: Cancer & Oncology Authors: Virginia Leiro Loretta De Chiara Mar Rodr íguez-Girondo Maribel Botana-Rial Diana Valverde Manuel N úñez-Delgado Alberto Fern ández-Villar Tags: Article Source Type: research
More News: Cancer | Cancer & Oncology | Cytology | Fine Needle Aspiration | Lung Cancer | Study | Ultrasound