Evaluation of the Modified Two-Tiered Testing Method for Diagnosis of Lyme Disease in Children Bacteriology

Conventional two-tiered testing (CTTT) for Lyme disease includes a first-tier enzyme immunoassay (EIA) followed by a supplemental immunoblot, and modified two-tiered testing (MTTT) relies on two different sequential EIAs without the inclusion of an immunoblot. MTTT has shown promising results as an alternative strategy for the diagnosis of Lyme disease in adults but has not yet been evaluated in children. We performed a cross-sectional study of children and adolescents ≤21 years of age undergoing clinical investigation for suspected Lyme disease. Serum specimens were analyzed with both a whole-cell sonicate (WCS) and a C6 EIA, with a supplemental immunoblot if either EIA was positive or equivocal. We compared CTTT (WCS EIA followed by supplemental immunoblot) to MTTT (WCS EIA followed by C6 EIA) using McNemar’s test to evaluate for agreement beyond chance alone. We then used a kappa statistic to measure level of the agreement between testing strategies. We included 1,066 serum specimens, of which 156 (14.6%) had a positive CTTT and 165 (15.5%) had a positive MTTT. There were no significant differences between MTTT and CTTT (P = 0.16). Although the overall agreement between MTTT and CTTT was high (kappa, 0.88; 95% confidence interval, 0.84 to 0.92), 33 children had discordant test results. In a cohort of children and adolescents undergoing investigation for suspected Lyme disease, CTTT and MTTT results agreed in most cases. Since immunoblots are time-consuming, labori...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research

Related Links:

Borrelia bavariensis is one of the agents of Lyme Borreliosis (or Lyme disease) in Eurasia. The genome of the Borrelia burgdorferi sensu lato species complex, that includes B. bavariensis, is known to be very com...
Source: BMC Genomics - Category: Genetics & Stem Cells Authors: Tags: Research article Source Type: research
The incidence of Lyme disease (LD) in North America has increased substantially in the past two decades. Concomitant with the increased incidence of infection has been an enhancement in the recognition of LD c...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Case report Source Type: research
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Conditions:   Lyme Disease;   Lyme Neuroborreliosis;   Lyme Arthritis;   Unknown Origin Fever Intervention:   Device: Scientific Consciousness Interface Operations (SCIO) Class II FDA approved medical device Sponsor:   Kasey Phifer Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
AbstractObjectivesThere is a lack of data on patients ’ and diagnostic factors for prognostication of complete recovery in patients with non-idiopathic peripheral facial palsy (FP).MethodsCohort register-based study of 264 patients with non-idiopathic peripheral FP and uniform diagnostics and standardized treatment in a university hospital from 2007 to 2017 (47% female, median age: 57  years). Clinical data, facial grading, electrodiagnostics, motor function tests, non-motor function tests, and onset of prednisolone therapy were assessed for their impact on the probability of complete recovery using univariable ...
Source: European Archives of Oto-Rhino-Laryngology - Category: ENT & OMF Source Type: research
(Natural News) You probably know that Lyme disease comes from ticks, but if you don’t personally suffer from it, you’d be forgiven for not knowing much more about it. However, there is one aspect of this disease that everyone should be concerned about, and that’s the possibility that it originated as a biological weapon developed...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
(Virginia Tech) Renowned tick immunobiologist Utpal Pal wants to adapt the rabies vaccination platform to produce antibodies that can protect against Borrelia burgdorferi, the bacteria responsible for Lyme disease. The intention is to apply this work to other tick-borne diseases in the future.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
The objective of the study was to determine the infection prevalence of Borrelia burgdorferi, a causative agent of Lyme disease, in the province's ticks and the seroprevalence in its dogs. It was found that 97.8% (n = 368) of ticks submitted were Ixodes scapularis, a species capable of transmitting Borrelia burgdorferi; 10.3% of these ticks [95% confidence interval (CI): 3.6% to 17.0%] were infected. Provincial canine seroprevalence for the 199 submitted samples was estimated at 3.0% (95% CI: 1.0% to 5.1%). PMID: 33012828 [PubMed - in process]
Source: The Canadian Veterinary Journal - Category: Veterinary Research Authors: Tags: Can Vet J Source Type: research
CONCLUSIONS: Lyme disease is rare, but must be kept in mind when investigating the etiology of chorioretinitis and retinal vasculitis. The patient reported here is, to our knowledge, the second case reported in literature that shows atypical clinic for Lyme disease with unilateral chorioretinitis without Erythema chronicum migrans (ECM). PMID: 32998513 [PubMed - as supplied by publisher]
Source: European Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Eur J Ophthalmol Source Type: research
Pharmacist Arlene Brailey continues to fight for better testing and diagnosis of Lyme disease, after a tick bite left her son chronically ill.
Source: The Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
More News: Children | Lyme Disease | Microbiology | Statistics | Study