Purification and identification of a novel antifungal protein from Bacillus subtilis XB-1

This study aimed to characterize a powerful antifungal component from bacteria.Bacillus subtilis strain XB-1, which showed maximal inhibition ofMonilinia fructicola, was isolated and identified, and an antifungal protein was obtained from it. Ammonium sulfate precipitation, ion exchange chromatography, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were used to purify and identify the proteins secreted byB. subtilis XB-1. Analyses revealed that purified fraction V had the strongest antifungal effect, with the largest pathogen inhibition zone diameter of 4.15  cm after 4 days (P <  0.05). This fraction showed a single band with a molecular weight of approximately 43 kDa in SDS-PAGE. Results from SDS-PAGE and liquid chromatography electrospray ionization tandem mass spectrometry analyses demonstrated that fraction V was likely a member of the chitosanase family. These resul ts suggest thatB. subtilis XB-1 and its antifungal protein may be useful in potential biocontrol applications.Graphic abstract
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research