Long-Distance Cooperative and Antagonistic RNA Polymerase Dynamics via DNA Supercoiling

Publication date: 19 September 2019Source: Cell, Volume 179, Issue 1Author(s): Sangjin Kim, Bruno Beltran, Irnov Irnov, Christine Jacobs-WagnerSummaryGenes are often transcribed by multiple RNA polymerases (RNAPs) at densities that can vary widely across genes and environmental conditions. Here, we provide in vitro and in vivo evidence for a built-in mechanism by which co-transcribing RNAPs display either collaborative or antagonistic dynamics over long distances (>2 kb) through transcription-induced DNA supercoiling. In Escherichia coli, when the promoter is active, co-transcribing RNAPs translocate faster than a single RNAP, but their average speed is not altered by large variations in promoter strength and thus RNAP density. Environmentally induced promoter repression reduces the elongation efficiency of already-loaded RNAPs, causing premature termination and quick synthesis arrest of no-longer-needed proteins. This negative effect appears independent of RNAP convoy formation and is abrogated by topoisomerase I activity. Antagonistic dynamics can also occur between RNAPs from divergently transcribed gene pairs. Our findings may be broadly applicable given that transcription on topologically constrained DNA is the norm across organisms.Graphical Abstract
Source: Cell - Category: Cytology Source Type: research

Related Links:

Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): L.C. Rhades, M. Larzábal, A. Bentancor, J. Sabio y García, F.J. Babinec, A. Cataldi, N. Amigo, V.N. Baldone, L. Urquiza, P.J. Delicia, M.C. FortAbstractBovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 15 October 2019Source: Microchemical JournalAuthor(s): Lu Liu, Guangying Zhao, Wenchao DouAbstractQuantitative and portable detection of target without skilled personnel and equipment is an important research focus in point-of-care testing field. A cost-effective, portable and unplugged foam immunochromatographic assay (Foam-ICA) for on-site and quantitative detection of Escherichia coli O157:H7 (E. coli O157:H7) without any instrument was reported. In sample solution the detection target E. coli O157:H7 were captured and labeled by monoclonal antibody modified the gold platinum bimetalli...
Source: Microchemical Journal - Category: Chemistry Source Type: research
Green Chem., 2019, Advance Article DOI: 10.1039/C9GC02286D, PaperBo Liang, Guannan Sun, Zhaobao Wang, Jian Xiao, Jianming Yang To biosynthesize 3-HP directly from malonate, a novel malonyl-CoA-mediated biosynthetic pathway was successfully developed. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Green Chem. latest articles - Category: Chemistry Authors: Source Type: research
We present two cases that HPeV was detected by multiplex polymerase chain reaction for respiratory viruses using bronchoalveolar lavage fluid and diagnosed as pneumonia caused by HPeV.
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
The objective of our present study was to evaluate the potency of the leaf extract of the plant Psidium guajava (PGE) along with its major biomarker quercetin against Shigella flexneri-induced sub chronic model of infectious diarrhoea. PGE at 100, 200 and 400 mg/kg, p.o. and quercetin at 50 mg/kg, p.o. were administered to Shigella flexneri-induced diarrhoeal rats for five days and various behavioural parameters were evaluated on 1st, 3rd and 5th day of treatment. This was followed by assessment of stool water content, density of Shigella flexneri in stools and blood parameters examination. After treatment, colon and s...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: January 2020Source: Microbial Pathogenesis, Volume 138Author(s): Momen Askoura, Mahmoud Youns, Wael Abdel Halim HegazyAbstractThe capacity of C. jejuni to survive acid and capture iron is a requirement for C. jejuni to colonize host and cause infection. Herein, we aimed to characterize the influence of iron on Campylobacter acid response. The capacity of C. jejuni to survive acid stress was greatly enhanced in presence of iron. Moreover, the acid stimulon of C. jejuni under iron-enriched condition was investigated using the microarray approach. A total of 211 genes were differentially expressed in C. jeju...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: December 2019Source: Microbial Pathogenesis, Volume 137Author(s): Faruq Abdulla, Utpal Kumar Adhikari, M. Kamal UddinAbstractTill now, AIDS, caused by the human immunodeficiency virus (HIV) is still a severe health problem worldwide. It weakens the immune system by targeting the T-helper cells. Specifically, the severity of the pandemic HIV-1 makes the emergence of an enduring effective vaccine against HIV-1. Therefore, we have applied a series of immunoinformatics approaches to the four conserved domains of HIV-1 integrase (IN) proteins to design an effective multi-epitope based subunit vaccine which mig...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
In this study, the promoter regions of 11 genes associated with contagious mastitis including CCL4, CXCL8, STAT3, IKBKB, MAPK14, NFKBIA, NFKB1, TNF, IL18, IL6, and HCK were investigated to predict the activating regulatory modules on promoters and to discover the key related transcription factors. By exploring the promoter regions, 228 genes were discovered comprising the same transcription factors modules. Out of 228 genes, 36 were validated using five microarray datasets. The promoter research of these genes revealed that as many as 7 down-regulated and 12 up-regulated genes are predictable in the network. The genes whos...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
In conclusion, imipenem and amikacin show good in vitro susceptibility rates against the IAI-ETP-NS-non-CPE isolates. The hepatobiliary ETP-NS-non-CPE displayed lower cefepime MICs than those cultured from the peritoneal space. Additionally, a significant fraction of IAI-ETP-NS-non-CP Enterobacter isolates exhibited ciprofloxacin MIC 
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Sepsis is a life-threatening condition associated with failure of at least one organ in the presence of infection. Along with SIRS, the acute systemic inflammatory syndrome without documented infection, sepsis represents a main health problem in intensive care units around the world. Hypercytokinemia and overexpression of activation-markers on leukocytes are frequently reported in SIRS/sepsis. Leukocyte functions including antibody mediated-phagocytosis, pathogen recognition, and migration appear to be disabled in SIRS/septic patients. Our aim was to evaluate the so-called activation immunophenotype and functions related t...
Source: Shock - Category: Emergency Medicine Tags: Online Articles Source Type: research
More News: Cytology | Environmental Health | Gastroenteritis | Genetics