Use of ammonium sulfite as a post-column derivatization reagent for rapid detection and quantification of aldehydes by LC-MS.

Use of ammonium sulfite as a post-column derivatization reagent for rapid detection and quantification of aldehydes by LC-MS. Talanta. 2020 Jan 01;206:120172 Authors: Hu YN, Chen D, Zhang TY, Ding J, Feng YQ Abstract Derivatization is frequently used in liquid chromatography-mass spectrometry (LC-MS) to improve the ionization characteristics of analytes, and facilitate their structural elucidation and quantification. However, the off-line derivatization process usually requires more analysis time. Therefore, the present work develops a novel LC/post-column derivatization (PCD)/MS (LC-PCD-MS) method for rapid detection of aldehydes in complex samples. The PCD method is based on a bisulfite addition reaction with aldehydes that can be easily conducted by mixing the LC elute with an (NH4)2SO3 solution via a tee. The resulting derivatives readily produce diagnostic HSO3- ions under collision induced dissociation (CID) in the negative ion mode of MS, even at an extremely low CID energy such as 1 eV, which can significantly improve the detection selectivity of aldehydes and reduce the occurrence of false positives. The limits of detection (LODs) of hexanal and heptanal were 3 nM and 2 nM, respectively. Moreover, good linearity in the concentration range of 0.01-1 μM for hexanal and heptanal was obtained with the determination coefficient (R2) being greater than 0.996 and the intra- and inter-day relative standard deviations (RSDs)...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research