Strategies for the discovery and engineering of enzymes for biocatalysis.

Strategies for the discovery and engineering of enzymes for biocatalysis.
Curr Opin Chem Biol. 2013 Apr;17(2):215-20
Authors: Davids T, Schmidt M, Böttcher D, Bornscheuer UT
Abstract
Protein engineering is the most important method to overcome the limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts to facilitate the design of mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, advanced high-throughput methods were developed using, for example, FACS analysis or microfluidic systems. These achievements significantly facilitate the tailor-made design of enzymes to make them suitable for industrial applications.
PMID: 23523243 [PubMed - in process]

Source: Current Opinion in Chemical Biology - April 1, 2013 Category: Biochemistry Authors: Davids T, Schmidt M, Böttcher D, Bornscheuer UT Tags: Curr Opin Chem Biol Source Type: research