Protein conjugation with genetically encoded unnatural amino acids.

Protein conjugation with genetically encoded unnatural amino acids.
Curr Opin Chem Biol. 2013 May 8;
Authors: Kim CH, Axup JY, Schultz PG
Abstract
The site-specific incorporation of unnatural amino acids with orthogonal chemical reactivity into proteins enables the synthesis of structurally defined protein conjugates. Amino acids containing ketone, azide, alkyne, alkene, and tetrazine side chains can be genetically encoded in response to nonsense and frameshift codons. These bio-orthogonal chemical handles allow precise control over the site and stoichiometry of conjugation, and have enabled medicinal chemistry-like optimization of the physical and biological properties of protein conjugates, especially the next-generation protein therapeutics.
PMID: 23664497 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - May 8, 2013 Category: Biochemistry Authors: Kim CH, Axup JY, Schultz PG Tags: Curr Opin Chem Biol Source Type: research