Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture

Publication date: Available online 6 September 2019Source: BiologicalsAuthor(s): Vinícius Augusto Simão, Caetano Pedro Evangelista-Ribeiro, Heloisa Brand, Lougan Lagass-Pereira, Laís Fernanda Marques, Pedro Henrique Benites-Aoki, Roseli Nunes da Silveira-Antunes, Aldo Tonso, João Tadeu Ribeiro-PaesAbstractAdipose-derived mesenchymal stromal/stem cells (ASC) have acquired a prominent role in tissue engineering and regenerative medicine. However, the standardization of basic culture procedures in this cellular type is still not well established according to the main qualitative cellular attributes. We evaluate the cell growth profile of human ASC in a different culture medium volumes and their nutritional composition utilizing static cultivation. Culture medium volumes (5, 10 and 15 mL/25 cm2) in T-flasks were evaluated by kinetic parameters and the metabolic composition was determined by biochemical analysis and Fourier transform infrared (FT-IR) absorption spectroscopy. 50% renewal of culture medium volume every 48 h was adopted. Immunophenotypic characterization and cell differentiation were performed. There was no difference (p > 0.05) in the kinetic parameters of cell proliferation between the culture medium volumes or in FT-IR composition. However, the concentrations of glucose, glutamine, lactate, and glutamate varied significantly during the cultivation process as a function of the medium volume. ASC presented specific antigens and differentiation potential ...
Source: Biologicals - Category: Biology Source Type: research