Calorespirometric Investigation of Streptococcus zooepidemicus Metabolism: Thermodynamics of Anabolic Payload Contribution by Growth and Hyaluronic Acid Synthesis

This study accomplished the HA production at different initial glucose concentrations, S0 (10 – 60 g/L) to subject different levels of anabolic burden on S. zooepidemicus. Anabolic payload comprising Biomass and HA yields showed a concomitant decrease with respect to the increased concentration of S0. Chemical entropy exported over the cell surface in the form of LA production exhibited an increasing trend at different levels of glucose, thus reducing the total yields of biomass and HA. Thermodynamically anabolic load contributed by biomass and HA production found to have minor influence over the driving force of S. zooepidemicus metabolism due to their lower yields. The entropy contribution to the overall driving force is significant (TΔSX=13 ΔrGX) at the higher biomass yields. This study allows the prediction of optimum biomass yield towards enhanced HA production and addresses the scope of ‘thermodynamic constraints’ application in real-time process monitoring and control using data reconciliation strategy in the near future.
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research