Inhibition of Agrobacterium tumefaciens biofilm formation by acylase I-immobilized polymer surface grafting of a zwitterionic group-containing polymer brush
Publication date: Available online 14 September 2019Source: Biochemical Engineering JournalAuthor(s): Qian Bao, Li Xie, Hidenori Ohashi, Masaaki Hosomi, Akihiko TeradaAbstractMembrane processes for water purification and wastewater treatment are impacted by biofilm formation, a hotspot for bacterial cell-cell communication, always use N-acyl homoserine lactones (AHLs) as signal compounds. Polymer materials for membrane filtration require high water permeability and biofilm deterrents. Therefore, in this study, a hydrophilic and enzyme-immobilizing polymer membrane was designed, for the first time, to prevent biofilm format...
Source: Biochemical Engineering Journal - September 15, 2019 Category: Biochemistry Source Type: research

Production and characterization of Hantaan Virus-Like Particles from Baculovirus Expression System
Publication date: Available online 14 September 2019Source: Biochemical Engineering JournalAuthor(s): Xiaoxia Dai, Can Jian, Li Na, Xiaofeng Wang, Yu Dai, Dexin LiAbstractHantaan virus-like particles (VLPs) have been produced and purified by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in baculovirus insect cell systems. The potential of VLPs as a vaccine was evaluated by comparing the humoral immune response and neutralizing antiserum induced by the purified VLP and the recombinant baculovirus bearing the Hantaan virus glycoproteins alone. After immunization of C57BL/6 mice, Hantaan V...
Source: Biochemical Engineering Journal - September 15, 2019 Category: Biochemistry Source Type: research

Effect of electrocoagulation on MBR under different power supply conditions
Publication date: Available online 13 September 2019Source: Biochemical Engineering JournalAuthor(s): Jiadong Liu, Xin Ju, Bo Gao, Lei WangAbstractThe impact of electrocoagulation (EC) on membrane bioreactor (MBR) under different power supply conditions was investigated in this study, which included constant voltage/current of 1 V, 3 V, 5 V and 0.15 A from direct current (DC) power source and undulate voltage from 18 V/10 W solar panel. The EC actuated by constant current of 0.15 A from DC power source and undulate voltage from 18 V/10 W solar panel could mitigate the membrane fouling obviously with 14 and 20 days of opera...
Source: Biochemical Engineering Journal - September 15, 2019 Category: Biochemistry Source Type: research

Optimisation of 2,3-butanediol production by Enterobacter ludwigii using sugarcane molasses
Publication date: Available online 12 September 2019Source: Biochemical Engineering JournalAuthor(s): Olga Psaki, Sofia Maina, Anestis Vlysidis, Seraphim Papanikolaou, Aline Machado de Castro, Denise M.G. Freire, Endrit Dheskali, Ioannis Kookos, Apostolis KoutinasAbstractFermentation media optimisation for 2,3-butanediol (BDO) production from sugarcane molasses with Enterobacter ludwigii has been conducted. Plackett-Burman design, central composite design and constrained optimisation were combined to optimise nutrient composition leading to enhanced BDO production efficiency. (NH4)2SO4, yeast extract and KOH were the most ...
Source: Biochemical Engineering Journal - September 13, 2019 Category: Biochemistry Source Type: research

Improving nitrogen removal in an IFAS nitritation–anammox reactor treating lagoon supernatant by manipulating biocarrier filling ratio and hydraulic retention time
Publication date: 15 December 2019Source: Biochemical Engineering Journal, Volume 152Author(s): Sen Yang, Shengnan Xu, Abdul Mohammed, Nicholas J. Ashbolt, Yang LiuAbstractA lab-scale integrated fixed-film activated sludge sequencing batch reactor (IFAS-SBR) was established for a one-stage nitritation–anammox process treating lagoon supernatant and optimized by altering both the biocarrier filling ratio and HRT for 500 days. The reactor’s nitrogen removal rate increased slightly from 0.25 kg N m−3 d−1 to 0.29 kg N m−3 d−1 when biocarrier filling ratio was increased from 35% t...
Source: Biochemical Engineering Journal - September 13, 2019 Category: Biochemistry Source Type: research

Carbon dioxide fixation by a hydrogen-oxidizing bacterium: biomass yield, reversal respiratory quotient, stoichiometric equations and bioenergetics
Publication date: Available online 9 September 2019Source: Biochemical Engineering JournalAuthor(s): Jian Yu, Yue LuAbstractA strain of Cupriavidus necator was grown on a gas mixture of hydrogen, oxygen and carbon dioxide to measure the cell mass formation and uptake rates of individual gases. The cell mass yield on consumed hydrogen and reversal respiratory quotient (RRQ) changed with cell density, indicating dynamic growth of the autotrophic microbes. A general stoichiometric equation was formulated by combining three half reactions of electron donor, electron accepter and cell mass synthesis. The only unknown parameter ...
Source: Biochemical Engineering Journal - September 10, 2019 Category: Biochemistry Source Type: research

Improving nitrogen removal in an IFAS nitritation-anammox reactor treating lagoon supernatant by manipulating biocarrier filling ratio and hydraulic retention time
Publication date: Available online 5 September 2019Source: Biochemical Engineering JournalAuthor(s): Sen Yang, Shengnan Xu, Abdul Mohammed, Nicholas J. Ashbolt, Yang LiuAbstractA lab-scale integrated fixed-film activated sludge sequencing batch reactor (IFAS-SBR) was established for a one-stage nitritation-anammox process treating lagoon supernatant and optimized by altering both the biocarrier filling ratio and HRT for 500 days. The reactor’s nitrogen removal rate increased slightly from 0.25 kg N m−3 d−1 to 0.29 kg N m−3 d−1 when biocarrier filling r...
Source: Biochemical Engineering Journal - September 6, 2019 Category: Biochemistry Source Type: research

Multistage Fluidized Bed Bioreactor for Dye Decolorization using Immobilized Polyurethane foam: A Novel Approach
Publication date: Available online 5 September 2019Source: Biochemical Engineering JournalAuthor(s): Shalini, Y. Pydi SettyAbstractA multistage fluidized bed bioreactor (M-FBBR) was developed for Congo Red (CR) dye removal using Polyurethane foam (PUF)-immobilized Bacillus subtilis. The active surface area for microbial growth and biological interaction are important factors to be considered for immobilization mediated decolorization. In the present study, the active surface area can be improved in the reactor in two ways, (i) by avoiding the accumulation of PUF on the top surface of reaction volume and (ii) by providing f...
Source: Biochemical Engineering Journal - September 6, 2019 Category: Biochemistry Source Type: research

Calorespirometric Investigation of Streptococcus zooepidemicus Metabolism: Thermodynamics of Anabolic Payload Contribution by Growth and Hyaluronic Acid Synthesis
This study accomplished the HA production at different initial glucose concentrations, S0 (10 – 60 g/L) to subject different levels of anabolic burden on S. zooepidemicus. Anabolic payload comprising Biomass and HA yields showed a concomitant decrease with respect to the increased concentration of S0. Chemical entropy exported over the cell surface in the form of LA production exhibited an increasing trend at different levels of glucose, thus reducing the total yields of biomass and HA. Thermodynamically anabolic load contributed by biomass and HA production found to have minor influence over the driving force...
Source: Biochemical Engineering Journal - September 6, 2019 Category: Biochemistry Source Type: research

Decellularized human amniotic membrane associated with adipose derived mesenchymal stromal cells as a bioscaffold: physical, histological and molecular analysis
Publication date: Available online 4 September 2019Source: Biochemical Engineering JournalAuthor(s): Liliana Sous Naasani, Aline Francielle Damo Souza, Cristiano Rodrigues, Samlai Vedovatto, Jéssica Gonçalves Azevedo, Ana Paula Santin Bertoni, Marilda Da Cruz Fernandes, Silvio Buchner, Márcia Rosângela WinkAbstractThe human amniotic membrane (hAM) has a great potential as a wound dressing and its combination with mesenchymal stromal cells (MSCs) represents an alternative therapy for wound healing. The scaffold should maintain the stemness of cells, so that they can present their immunomodulatory ...
Source: Biochemical Engineering Journal - September 5, 2019 Category: Biochemistry Source Type: research

Homoethanol production from cellobionate and glycerol using recombinant Klebsiella oxytoca strains
Publication date: Available online 3 September 2019Source: Biochemical Engineering JournalAuthor(s): Weiyi Tao, Takao Kasuga, Shuang Li, He Huang, Zhiliang FanAbstractCellobionic acid, an oxidized cellulose degradation product, can be produced from cellulosic biomass. Engineered strains of Klebsiella oxytoca BW21 and WT26 can effectively use cellobionate for ethanol production. However, because cellobionate is a more oxidized substrate than cellobiose, oxidized products such as acetate must be produced as by-products to maintain the overall redox balance, and homoethanol production is not possible. One of the strategies to...
Source: Biochemical Engineering Journal - September 4, 2019 Category: Biochemistry Source Type: research

Construction and characterization of bifunctional cellulases: Caldicellulosiruptor-sourced endoglucanase, CBM, and exoglucanase for efficient degradation of lignocellulose
In conclusion, lignocellulose saccharification ability, high pH and temperature stability, make the CBMs-fused bifunctional cellulases attractive candidate for natural substrates deconstruction. (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - September 1, 2019 Category: Biochemistry Source Type: research

Concomitant consumption of glucose and lactate: A novel batch production process for CHO cells
Publication date: Available online 31 August 2019Source: Biochemical Engineering JournalAuthor(s): I. Martínez-Monge, P. Comas, J. Triquell, A. Casablancas, M. Lecina, C. Paredes, J.J. CairóAbstractThe rapid consumption of large quantities of glucose in conventional CHO batch processes yields large quantities of lactate, leading to the inhibition of cell growth. We have observed that under certain culture conditions, CHO cells are able to co-metabolize glucose and lactate, even during the growth phase.Taking advantage of this metabolic behavior, a new batch process has been defined where there is a concomitan...
Source: Biochemical Engineering Journal - September 1, 2019 Category: Biochemistry Source Type: research

Editorial – Bioprocess Development
Publication date: Available online 29 August 2019Source: Biochemical Engineering JournalAuthor(s): James Winterburn (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - August 31, 2019 Category: Biochemistry Source Type: research

Colin Webb: The man, the mentor and the microbes
Publication date: Available online 29 August 2019Source: Biochemical Engineering JournalAuthor(s): Grant Campbell (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - August 31, 2019 Category: Biochemistry Source Type: research

A Rapid Method to Quantify the Biomass of Viable Acidithiobacillus ferrooxidans in Iron-based Bioleaching Matrix of Sewage Sludge
Publication date: Available online 30 August 2019Source: Biochemical Engineering JournalAuthor(s): Decheng Jin, Lanlan Liu, Guanyu Zheng, Jianru Liang, Lixiang ZhouAbstractOverlay plate counting is the traditional way to determine the biomass of viable Acidithiobacillus ferrooxidans in iron-based bioleaching matrix of sewage sludge. However, the time required is 12 days at a minimum. Here, a rapid method based on a quantitative relationship between the extant rate of iron bio-oxidation by Acidithiobacillus ferrooxidans and the CFU counts was developed. The method reduces the time to ∼3 h. In the new method, the ...
Source: Biochemical Engineering Journal - August 31, 2019 Category: Biochemistry Source Type: research

Adaptation of Cupriavidus necator to levulinic acid for enhanced production of P(3HB-co-3HV) copolyesters
Publication date: 15 November 2019Source: Biochemical Engineering Journal, Volume 151Author(s): Ivana Novackova, Dan Kucera, Jaromir Porizka, Iva Pernicova, Petr Sedlacek, Martin Koller, Adriana Kovalcik, Stanislav ObrucaAbstractEvolutionary engineering is an approach, which allows preparing microorganisms with desired phenotype without the need for knowledge of genetic characteristics connected with changes at phenotype level. This approach is based on cell multiplication under stress conditions and it can be performed in various designs. In connection with PHA-producing bacteria, evolutionary engineering can be used to g...
Source: Biochemical Engineering Journal - August 28, 2019 Category: Biochemistry Source Type: research

Impact of Oxidative Stress on Protein Production by Bordetella pertussis for Vaccine Production
Publication date: Available online 23 August 2019Source: Biochemical Engineering JournalAuthor(s): Vanessa Zavatti, Hector Budman, Raymond L. Legge, Melih TamerAbstractThe response of Bordetella pertussis to induced oxidative stress during fermentation was assessed. B. pertussis is the causal agent of whooping cough in humans and purified pertactin (PRN) is used as one of the components in the production of acellular pertussis vaccines. When grown in bioreactors, bacteria adapt to environmental conditions which may include high levels of reactive oxidative species (ROS) that stress cells and can have detrimental effects on...
Source: Biochemical Engineering Journal - August 25, 2019 Category: Biochemistry Source Type: research

Statistical and optimization strategies to carotenoids production by Tetradesmus acuminatus (LC192133.1) cultivated in photobioreactors
Publication date: Available online 23 August 2019Source: Biochemical Engineering JournalAuthor(s): Vanessa Daneluz Gonçalves, Marcia Regina Fagundes Klen, Daniela Estelita Goes Trigueros, Alexander Dimitrov Kroumov, Aparecido Nivaldo MódenesAbstractIn this work, the effects of the factors light intensity, photoperiod, pH, NaCl and nitrogen concentration were investigate on the carotenoids response of Tetradesmus acuminatus (LC192133.1) cultures. Analysis of variance (α = 0.10) suggested significant effect mainly of the light intensity and photoperiod factors. The applied regression models,...
Source: Biochemical Engineering Journal - August 25, 2019 Category: Biochemistry Source Type: research

Understanding the biocalorimetric and respirometric behaviour of co-culture (R. eutropha, P. putida and A. vinelandii) in poly (3-hydroxybutyrate) batch production
Publication date: Available online 7 August 2019Source: Biochemical Engineering JournalAuthor(s): Anusha Subramanian Mohanakrishnan, Sivanesh Nanjan Easwaran, Deepa Perinkulam Ravi, Surianarayanan MahadevanAbstractLimiting nitrogen and excess carbon sources induce accumulation of the bioplastic, Poly-(3-hydroxybutyrate) (PHB) as inclusion bodies in microbes. Here, we designed a co-culture (R. eutropha, P.putida, A.vinelandii) that produced higher PHB yield than in monocultures or pairwise combination. PHB concentration of 8.3 ± 0.42 g/L, which is 63% of total Cell Dry Mass (CDM), was achieved us...
Source: Biochemical Engineering Journal - August 22, 2019 Category: Biochemistry Source Type: research

Enhancing the performance of E. coli-powered MFCs by using porous 3D anodes based on coconut activated carbon
In this study, activated carbon felt (C3P1AC1) fabricated by combining coconut-shell activated carbon powder (CSAC), carbon fiber, and cellulose fiber was used as the anode electrode in an Escherichia coli (E. coli) biofilm-inoculated MFC. CSAC ratio in the C3P1AC1 anodes was varied from 0 to 75 wt%, called C3P1AC1-X (X = 0, 10, 20, 40, 60, and 75), to investigate the effectiveness of CSAC on the performance of the anode. The C3P1AC1 anodes were characterized by XRD, SEM, EDS, and sheet resistance measurement. The maximum power density of the MFC has increased 7.2-fold from 0.53μWcm-2 when using the...
Source: Biochemical Engineering Journal - August 22, 2019 Category: Biochemistry Source Type: research

Dynamic simulation of N2O emissions from a full-scale partial nitritation reactor
This study deals with the potential and the limitations of dynamic models for describing and predicting nitrous oxide (N2O) emissions associated with biological nitrogen removal from wastewater. The results of a three-week monitoring campaign on a full-scale partial nitritation reactor were reproduced through a state-of-the-art model including different biological N2O formation pathways. The partial nitritation reactor under study was a SHARON reactor treating the effluent from a municipal wastewater treatment plant sludge digester. A qualitative and quantitative comparison between experimental data and simulation results ...
Source: Biochemical Engineering Journal - August 22, 2019 Category: Biochemistry Source Type: research

Quantitative detection of viable Escherichia coli O157:H7 using a photoreactive DNA-binding dye propidium monoazide in irrigation water
In this study, a quantitative PCR (qPCR) assay with propidium monoazide (PMA) treatment called PMA-qPCR assay was developed to detect the presence of E. coli O157:H7 in natural irrigation water that was more specific, more sensitive, and was unaffected by dead cells compared with traditional and conventional methods. Specific gene, fliC from E. coli O157:H7 was selected to design the unique primer and probe for the assay while propidium monoazide (PMA) was used to effectively prevent the interference of DNA from dead cells. Evaluation of the effect of PMA treatment exhibited permeation of the dead cell membranes that was o...
Source: Biochemical Engineering Journal - August 20, 2019 Category: Biochemistry Source Type: research

Effects of energy source on bioleaching of vanadium-bearing shale by Acidithiobacillus ferrooxidans
Publication date: Available online 19 August 2019Source: Biochemical Engineering JournalAuthor(s): Jingtao He, Zhenlei Cai, Yimin Zhang, Nannan Xue, Xingjie Wang, Qiushi ZhengAbstractThe effects of energy source (ferrous iron, elemental sulfur and its mixture) on bioleaching of differently pretreated vanadium-bearing shale by Acidithiobacillus ferrooxidans (A. ferrooxidans) were investigated in this paper. The results in the absence of energy source indicated that bioleaching efficiencies of vanadium from raw ore and decarbonized sample were 25.32% and 30.11%, respectively. The positive effects of sulfur as a sole energy s...
Source: Biochemical Engineering Journal - August 20, 2019 Category: Biochemistry Source Type: research

Identification of the Regulon FkbN for Ascomycin Biosynthesis and its Interspecies Conservation Analysis as LAL Family Regulator
Publication date: Available online 17 August 2019Source: Biochemical Engineering JournalAuthor(s): Yue Zhang, Hong Chen, Pan Wang, Jianping WenAbstractAscomycin is a 23-membered polyketide macrolide with high immunosuppressant activity, produced by Streptomyces hygroscopicus var. ascomyceticus. However, the specific regulatory mechanism of the ascomycin biosynthesis pathway remains poorly understood. Here, we show that FkbN is a pathway-specific positive regulator for the ascomycin biosynthesis. Inactivation of fkbN led to a complete loss of ascomycin production ability. Compared with the parental strain, the ascomycin yie...
Source: Biochemical Engineering Journal - August 19, 2019 Category: Biochemistry Source Type: research

The symbiosis of anaerobic ammonium oxidation bacteria and heterotrophic denitrification bacteria in a size-fractioned single-stage partial nitrification/anammox reactor
In this study, the symbiosis of heterotrophic denitrification bacteria (HDB) and anaerobic ammonium oxidation bacteria (AAOB) was observed in a size-fractioned single-stage partial nitrification/anammox reactor to explore their individual contribution. The reactor was operated in three phases, with different aeration rates (0.6, 0.4, and 0.5 L·min-1) for 73 days. In the third phase, a maximum nitrogen removal efficiency (NRE) of 92.0% was achieved and the nitrogen removal rate (NRR) was 0.498 kg N·m-3·d-1. A high special anammox activity (SAA) of 227.4 mg N·g-1...
Source: Biochemical Engineering Journal - August 19, 2019 Category: Biochemistry Source Type: research

A comparison of methods of ethanol production from sweet sorghum bagasse
Publication date: Available online 17 August 2019Source: Biochemical Engineering JournalAuthor(s): Anusith Thanapimmetha, Maythee Saisriyoot, Chanin Khomlaem, Yusuf Chisti, Penjit SrinophakunAbstractEthanol production from sweet sorghum bagasse (SSB) is reported. A two-step pretreatment with hydrogen peroxide followed by NaOH at 121 °C for 20 min, was used prior to enzymatic treatment. Enzymatic treatment was carried out using a commercial cellulase. Enzymatic hydrolysis for 72 h resulted in a hydrolysate containing xylose (5.1 g L―1), glucose (75.3 g L―1) and cellobiose (1.4&thin...
Source: Biochemical Engineering Journal - August 19, 2019 Category: Biochemistry Source Type: research

Adaptation of cupriavidus necator to levulinic acid for enhanced production of P(3HB- co -3HV) copolyesters
Publication date: Available online 16 August 2019Source: Biochemical Engineering JournalAuthor(s): Ivana Novackova, Dan Kucera, Jaromir Porizka, Iva Pernicova, Petr Sedlacek, Martin Koller, Adriana Kovalcik, Stanislav ObrucaAbstractEvolutionary engineering is an approach, which allows preparing microorganisms with desired phenotype without the need for knowledge of genetic characteristics connected with changes at phenotype level. This approach is based on cell multiplication under stress conditions and it can be performed in various designs. In connection with PHA-producing bacteria, evolutionary engineering can be used t...
Source: Biochemical Engineering Journal - August 17, 2019 Category: Biochemistry Source Type: research

Effects of CaCl2 freeze-drying and acidic solutions on the reusability of calcium alginate beads; and degradation of phenol by immobilized Acinetobacter sp. PR1
Publication date: Available online 15 August 2019Source: Biochemical Engineering JournalAuthor(s): Li Xia, Xiaotong Luo, Yunfei Zhu, Xiang Zhang, Lixin LuoAbstractApplications of immobilization technology to wastewater treatment have achieved mixed results, that is, high degradation efficiency or good reusability. In this research, alginate was used as supporting material of immobilized beads to maintain high degradation efficiency. Applying CaCl2 freeze drying and acidic solutions led to the reusability of the beads. Ca-alginate beads can be reused for more than 21 cycles of repeated CaCl2 freeze drying and 16 cycles of t...
Source: Biochemical Engineering Journal - August 17, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 15 October 2019Source: Biochemical Engineering Journal, Volume 150Author(s): (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - August 17, 2019 Category: Biochemistry Source Type: research

BEJ Young Investigator Award
Publication date: 15 October 2019Source: Biochemical Engineering Journal, Volume 150Author(s): (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - August 17, 2019 Category: Biochemistry Source Type: research

Triticum spelta straw hydrothermal pretreatment for the production of glucose syrups via enzymatic hydrolysis
Publication date: Available online 14 August 2019Source: Biochemical Engineering JournalAuthor(s): Rodrigo da Rocha Olivieri de Barros, Paolo Becarelli, Raul Alves de Oliveira, Leonardo Tognotti, Elba Pinto da Silva BonABSTRACTThe global climate changes related to the use fossil fuels and the oil price volatility have been feeding the interest for renewable feedstock such as the by-products of the agroindustry. This is a challenging scenario as the diversity of plant materials worldwide calls for customized pretreatment processes to achieve a significant cellulose enzymatic saccharification coupled to the preservation of t...
Source: Biochemical Engineering Journal - August 16, 2019 Category: Biochemistry Source Type: research

Cultivation modes for microbial oil production using oleaginous yeasts – A review
Publication date: Available online 14 August 2019Source: Biochemical Engineering JournalAuthor(s): Eleni E. Karamerou, Colin WebbAbstractMicrobial oil has been a valuable alternative oil source with applications in the food, cosmetics and biofuels sectors. As concerns for the environment grow, microbially-synthesized oil has emerged as a potential contributor for sustainable production of biodiesel. However, the high costs of its production hinder its large-scale application. Main bottleneck to industrial lipid production is the cost of the fermentation stage. Therefore, it is imperative to design cultivations with little ...
Source: Biochemical Engineering Journal - August 16, 2019 Category: Biochemistry Source Type: research

Metabolic modeling of bacterial co-culture systems predicts enhanced carbon monoxide-to-butyrate conversion compared to monoculture systems
Publication date: Available online 13 August 2019Source: Biochemical Engineering JournalAuthor(s): Xiangan Li, Michael A. HensonAbstractWe used metabolic modeling to computationally investigate the potential of bacterial coculture system designs for CO conversion to the platform chemical butyrate. By taking advantage of the native capabilities of wild-type strains, we developed two anaerobic coculture designs by combining Clostridium autoethanogenum for CO-to-acetate conversion with bacterial strains that offer high acetate-to-butyrate conversion capabilities: the environmental bacterium Clostridium kluyveri and the human ...
Source: Biochemical Engineering Journal - August 14, 2019 Category: Biochemistry Source Type: research

Strengthening of Aerobic Sludge Granulation by the Endogenous Acylated Homoserine Lactones-Secreting Strain Aeromonas sp. A-L3
Publication date: Available online 12 August 2019Source: Biochemical Engineering JournalAuthor(s): Min Gao, Yongjun Liu, Zhe Liu, Haitao Li, Aining ZhangAbstractIncreasing the quorum sensing effect has become an effective means of strengthening the sludge granulation process in recent years, but most of the current methods for achieving this effect involve the direct addition of signal molecules. To identify a more environmentally friendly biological method for accelerating the formation of aerobic granular sludge (AGS) and further reducing the operating cost, an Aeromonas sp. strain (A-L3) screened from AGS seed sludge wa...
Source: Biochemical Engineering Journal - August 13, 2019 Category: Biochemistry Source Type: research

Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
Publication date: Available online 12 August 2019Source: Biochemical Engineering JournalAuthor(s): Zuharlida Tuan Harith, Dimitris Charalampopoulos, Afroditi ChatzifragkouAbstractRapeseed meal, a by-product of oil processing industry, was evaluated as a substrate for astaxanthin production by the yeast Xanthophyllomyces dendrorhous DSMZ 5626. Four commercial enzymes were tested at different concentrations (1–15%, v/v) for their ability to break down the cellulosic and hemicellulosic compounds of rapeseed meal into fermentable sugars. Viscozyme® L and cellulase demonstrated the highest glucose recovery yields (47&...
Source: Biochemical Engineering Journal - August 13, 2019 Category: Biochemistry Source Type: research

Inference of dynamic macroscopic models of cell metabolism based on elementary flux modes analysis
This study aims at providing a methodology based on both data- and knowledge-driven approaches to build dynamic macroscopic models of cell cultures. This methodology proceeds in three steps. A principal component analysis is first applied in order to determine the minimum number of macroreactions necessary to faithfully describe the available data. These reactions are then selected among the elementary flux modes associated with a chosen metabolic network through the definition of an original linear programming problem. Kinetic laws are finally identified so as to reproduce the measurement data. The proposed methodology is...
Source: Biochemical Engineering Journal - August 13, 2019 Category: Biochemistry Source Type: research

Nitrogen flow and microbial community in the anoxic reactor of “Sulfate Reduction, Denitrification/Anammox and Partial Nitrification” process
Publication date: Available online 11 August 2019Source: Biochemical Engineering JournalAuthor(s): Yuka Kosugi, Norihisa Matsuura, Qiaochu Liang, Ryoko Yamamoto-IkemotoAbstractWe propose a sulfate reduction, denitrification/anammox and partial nitrification process to treat organic wastewater. Nitrogen removal in the anoxic reactor in this process was examined using an anaerobic-anoxic biofilter reactor. When nitrate medium was added to the anoxic reactor simulating the return flow from a nitrification reactor, mainly heterotrophic and sulfur denitrification occurred. However, anammox as well as denitrification occurred in...
Source: Biochemical Engineering Journal - August 13, 2019 Category: Biochemistry Source Type: research

Salt-enhanced permeabilization for monoclonal antibody precipitation and purification in a tubular reactor with a depth filtration membrane with advanced chromatin extraction
In this study, antibody purification was first achieved by precipitation of the antibody from the cell culture supernatant (CCS) in a tubular reactor with a static mixer. The protein precipitates were efficiently intercepted by incorporating a depth filtration membrane directly after the mixer. Chromatin-directed cell culture clarification was shown to enable the full usage of the load of the filter membrane and also significantly reduced the burden of the subsequent purification step. The addition of NaCl to the CCS significantly increased the permeabilization of the filter membrane by increasing the loading capacity by u...
Source: Biochemical Engineering Journal - August 11, 2019 Category: Biochemistry Source Type: research

Combined strategies for engineering a novel whole-cell biocatalyst of Candida rugosa lipase with improved characteristics
In this study, the optimized lipase 1 gene from Candida rugosa (CRL1) was displayed, for the first time, on the cell wall of Pichia pastoris using a truncated flocculation functional domain of Flo1p (FS) anchor. The hydrolytic activity of displayed CRL1 reached 7517 U/g under the control of the AOX1 promoter. Higher lipase activity, 19,771 U/g (an increase of 163%), was obtained by a five-copy CRL1 construct overexpressing the HAC1 factor. Flow cytometry and quantitative analysis demonstrated the CRL1 was successfully displayed on the yeast surface with high efficiency. Displayed CRL1 was characterized by the optimal tempe...
Source: Biochemical Engineering Journal - August 10, 2019 Category: Biochemistry Source Type: research

Determining uncertainties in PICRUSt analysis – an easy approach for autotrophic nitrogen removal
Publication date: Available online 8 August 2019Source: Biochemical Engineering JournalAuthor(s): Shelesh Agrawal, Co Thi Kinh, Thomas Schwartz, Masaaki Hosomi, Akihiko Terada, Susanne LacknerAbstractThe diversity and dynamics of microorganisms in engineered ecosystems have a high impact on performance and operational stability. Nitrogen removal from wastewater is one example of such complex and dynamic ecosystems. Following the microbial community composition and its functional potential is highly valuable information for optimizing performance. Molecular methods and data analysis tools have become more and more popular i...
Source: Biochemical Engineering Journal - August 9, 2019 Category: Biochemistry Source Type: research

Conflicting carbon footprint and energy saving in a side-stream Anammox Process
In this study, a pilot-scale Anammox reactor has been incorporated into an optimized full-scale wastewater treatment plant. The supernatant of the reject sludge waters has been treated by the full scale wastewater treatment plant with and without incorporating a full-scale Anammox reactor in the sludge line. Energy consumption and nitrous oxide emissions have been monitored in both scenarios.In terms of total impact, a side-stream Anammox process can effectively mitigate the nitrogen rich sludge reject water. The impact on the carbon footprint of the full-scale wastewater treatment plant is instead, to its best, neutral. T...
Source: Biochemical Engineering Journal - August 9, 2019 Category: Biochemistry Source Type: research

New approach for biological synthesis of reduced graphene oxide
Publication date: Available online 8 August 2019Source: Biochemical Engineering JournalAuthor(s): Carolina Vargas, Raquel Simarro, José Alberto Reina, Luis Fernando Bautista, María Carmen Molina, Natalia González-BenítezAbstractGraphene synthesis has created great interest because of the potential applications in various fields. Many works of graphene synthesis from chemical reduction of graphene oxide involving harmful reactants have been published. A battery of both aerobic and anaerobic gram-negative and gram-positive strains as well as an extremophile microbial consortium from natural enviro...
Source: Biochemical Engineering Journal - August 9, 2019 Category: Biochemistry Source Type: research

Enhancing the activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution and molecular dynamics simulation
This study improved the understanding of structure-activity relationship for MTG adapted to high temperature conditions. It also provides theoretical foundation and preliminary information for engineering MTG with enhanced characteristics to meet the industrial requirements. (Source: Biochemical Engineering Journal)
Source: Biochemical Engineering Journal - August 9, 2019 Category: Biochemistry Source Type: research

Understanding the biocalorimeteric and respirometric behaviour of Co-Culture (R. eutropha, P. putida and A. vinelandii) in Poly (3-hydroxybutyrate) batch production
Publication date: Available online 7 August 2019Source: Biochemical Engineering JournalAuthor(s): Anusha Mohanakrishnan Subramanian, Sivanesh Easwaran Nanjan, Deepa Perinkulam Ravi, Surianarayanan MahadevanAbstractLimiting nitrogen and excess carbon sources induce accumulation of the bioplastic, Poly-(3-hydroxybutyrate) (PHB) as inclusion bodies in microbes. Here, we designed a co-culture (R. eutropha, P.putida, A.vinelandii) that produced higher PHB yield than in monocultures or pairwise combination. PHB concentration of 8.3 ± 0.42 g/L, which is 63 % of total Cell Dry Mass (CDM), was achieved u...
Source: Biochemical Engineering Journal - August 9, 2019 Category: Biochemistry Source Type: research

Effects of C/N Ratio and DO concentration on Carbon and Nitrogen removals in a Hybrid Biological Reactor
The objective of this paper was to evaluate the effects of dissolved oxygen (DO) concentration and C/N ratio on the biological removal of carbon and nitrogen using an Integrated Fixed-film Activated Sludge (IFAS) reactor for nearly half a year. The results showed that the efficiency of organic matter removal was greater than 90% regardless of the value of the C/N ratio. Nitrogen removal was affected by the C/N ratio. Indeed, at a C/N ratio equal to 10, nitrogen removal efficiency was maximum with an average removal of 96.54%, 86.1% for ammonium and total nitrogen, respectively. Conversely, with a C/N ratio of 4, removal ...
Source: Biochemical Engineering Journal - August 4, 2019 Category: Biochemistry Source Type: research

Continuous controlled long-term operation and modeling of a closed loop connecting an air-lift photobioreactor and an animal compartment for the development of a life support system
Publication date: Available online 2 August 2019Source: Biochemical Engineering JournalAuthor(s): Laura Alemany, Enrique Peiro, Carolina Arnau, David Garcia, Laurent Poughon, Jean-François Cornet, Claude-Gilles Dussap, Olivier Gerbi, Brigitte Lamaze, Christophe Lasseur, Francesc GodiaAbstractThe MELiSSA (MicroEcological Life Support System Alternative) project is developing a closed regenerative life support system for long-term space missions by considering the integration of six biological compartments tested in a dedicated pilot plant. The technological effort of building such a closed and sustainable ecological ...
Source: Biochemical Engineering Journal - August 3, 2019 Category: Biochemistry Source Type: research

A minimalist peptide ligand for IgG by minimizing the binding domain of protein A
This study presents a strategy to design a short peptide ligand by minimizing the IgG binding portion from a commercial ligand of Protein A. A short hexapeptide (FYEILH) was thus screened out by the real screening technique of STD-NMR. It presented a comparable binding activity to Protein A, with the dissociation constant (Kd) of 1.8 × 10-6 mol/L, the static binding capacity of 49.7 mg/mL, and the purity of 94% for hIgG from chromatographic purification of serum feedstock in a single step. Epitope mapping analysis by STD-NMR indicated that the carboxy groups of the charged residue E (glutamic aci...
Source: Biochemical Engineering Journal - August 3, 2019 Category: Biochemistry Source Type: research

Production of poly (3-hydroxybutyrate) from a dairy industry wastewater using Bacillus subtilis EPAH18: Bioprocess development and simulation
Publication date: Available online 31 July 2019Source: Biochemical Engineering JournalAuthor(s): Erika Peña-Jurado, Samuel Pérez-Vega, Francisco Javier Zavala-Díaz de la Serna, Ildebrando Pérez-Reyes, Nestor Gutiérrez-Méndez, José Vazquez-Castillo, Ivan SalmerónAbstractThe production of biopolymers such as poly (3-hydroxybutyrate) (PHB) from renewable resources has gained revived attention; although, fermenting media cost affects the production price. Whilst cheese whey (CW) is an alternative source of nutrients it is managed as wastewater in rural dairy factories. Th...
Source: Biochemical Engineering Journal - August 1, 2019 Category: Biochemistry Source Type: research

An electrochemical troponin I peptisensor using a triangular icicle-like gold nanostructure
Publication date: Available online 31 July 2019Source: Biochemical Engineering JournalAuthor(s): M. Negahdary, H. HeliAbstractDetection of troponin I (TnI), as a unique biomarker for myocardial infarction (MI), is highly important for diagnosis, prevention and therapy of MI, a major problem in the health policy and death. A triangular icicle-like gold nanostructure was firstly electrodeposited on a gold substrate to attain a high-surface area transducer, and characterized by field emission scanning electron microscopy. A 13-amino acid affinity peptide was then immobilized on the gold nanostructure surface to fabricate a Tn...
Source: Biochemical Engineering Journal - August 1, 2019 Category: Biochemistry Source Type: research