Sandwich antibody-based biosensor system for identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria.

In this study, we aim to establish an antibody-based biosensor system for the identification of MTBC and NTM infection. Monoclonal antibodies (mAbs) specific for Ag85B proteins of mycobacteria were generated and characterized. The generated anti-Ag85B mAb clones AM85B-5 and AM85B-8 reacted to Ag85B of Mycobacterium spp.; in contrast, clone AM85B-9 specifically reacted to Ag85B of MTBC. By employing the produced mAbs, single and sandwich antibody-based biosensors using bio-layer interferometry were established for determination of Ag85B proteins. The sandwich antibody-based biosensor system was demonstrated to be suitable for detection of Ag85B protein and identification of MTBC and NTM. Using anti-Ag85B mAbs AM85B-8 and AM85B-9 as immobilized antibodies on sensor chips and using mAb AM85B-5 as secondary antibody, the established sandwich antibody-based biosensor could discriminate MTBC and NTM. The developed biosensor system can be used for culture confirmation of mycobacteria and speciation to MTBC and NTM. PMID: 31462139 [PubMed - as supplied by publisher]
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Related Links:

Publication date: Available online 31 March 2020Source: Microbial PathogenesisAuthor(s): Jôsimar D. Moreira, Henrique Rodrigues Silva, Vicente de P.C.P. de Toledo, Tânia M.P.D. Guimarães
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 31 March 2020Source: Preventive Veterinary MedicineAuthor(s): Tiny Motlatso Hlokwe, Marcia Mogano Ratanna
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
This report provides an overview of the latest tuberculosis (TB) epidemiological situation in Europe.
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
CONCLUSION: Evaluation of immune status based on PD-L1 pleural concentration and Th2 to Th1 ratio might predict the risk of MDR-TB occurrence. PMID: 32224537 [PubMed - in process]
Source: Iranian Journal of Immunology - Category: Allergy & Immunology Tags: Iran J Immunol Source Type: research
[Daily Maverick] Covid-19 has been declared a pandemic by the World Health Organisation. In South Africa, multiple cases and several deaths have been confirmed. While the fight to contain the pandemic continues, a major area of concern is the additional vulnerability of those with TB and HIV to Covid-19, particularly those who are not on treatment.
Source: AllAfrica News: Tuberculosis - Category: Infectious Diseases Source Type: news
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLES Source Type: research
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLES Source Type: research
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLES Source Type: research
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLES Source Type: research
Source: International Journal of Tuberculosis and Lung Disease - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLES Source Type: research
More News: Biochemistry | Study | Tuberculosis