High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
Methods Mol Biol. 2020;2052:319-334
Authors: Zhang H, Zhu G
Abstract
An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.
PMID: 31452170 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Zhang H, Zhu G Tags: Methods Mol Biol Source Type: research