NK2 receptor mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent calcium channels and the RhoA-Rho kinase pathway.

NK2 receptor mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent calcium channels and the RhoA-Rho kinase pathway. Am J Physiol Renal Physiol. 2019 Aug 28;: Authors: Dér B, Molnár PJ, Ruisanchez É, Őrsy P, Kerék M, Faragó B, Nyirády P, Offermanns S, Benyó Z Abstract Tachykinins (TKs) are involved both in the physiological regulation of urinary bladder functions and in the development of overactive bladder (OAB) syndrome. The aim of the present study was to investigate the signal transduction pathways of TKs in the detrusor muscle to provide potential pharmacological targets for the treatment of bladder dysfunctions related to enhanced TK production. Contraction force, intracellular Ca2+ concentration([Ca2+]ic) and RhoA activity were measured in mouse urinary bladder smooth muscle (UBSM). TKs and the NK2 receptor (NK2R) specific agonist [β-Ala⁸]-NKA(4-10) evoked contraction, which was inhibited by the NKR2 antagonist MEN10376. In Gαq/11 deficient mice [β-Ala⁸]-NKA(4-10)-induced contraction and [Ca2+]ic increase were abolished. Although Gq/11-proteins are linked principally to phospholipase C β (PLC-β) and inositol trisphosphate-mediated Ca2+ release from intracellular stores, we found that PLC-β inhibition and sarcoplasmic reticulum Ca2+ depletion failed to have any effect on contraction induced by [β-Ala⁸]-NKA(4-10). In co...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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Scientists at the University of Portsmouth are a step closer in the development of a 'game-changer' test which will diagnose an overactive bladder quicker, slowing the condition's progression.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
AbstractOveractive bladder (OAB) is a common condition affecting one ‐sixth to one‐fifth of the global population. The treatment of refractory OAB remains a challenge for urologists. Current treatment options include the use of combination therapy with antimuscarinic agents and beta‐3 adrenoceptor agonists, and treating underlying curable disorders. Intravesica l botulinum toxin type A (BoNT‐A) injection, percutaneous tibial nerve stimulation, and sacral nerve stimulation are third‐line management therapies suggested by the American Urological Association/Society of Urodynamics, Female Pelvic Medicine&Urogeni...
Source: LUTS: Lower Urinary Tract Symptoms - Category: Urology & Nephrology Authors: Tags: REVIEW ARTICLE Source Type: research
People with an overactive bladder could have a rechargeable wireless device fitted to ease their condition, NICE has said in new draft guidance.
Source: NHS Networks - Category: UK Health Source Type: news
This study demonstrates positive effects of combination drug therapy on symptoms of OAB, possibly indicating it to be a good option for treatment of OAB during concomitant cystitis. It remains to be determined if increased release of NO is crucial for successful pharmacological treatment of bladder overactivity during cystitis.
Source: Pharmacology Research and Perspectives - Category: Drugs & Pharmacology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Despite adequate management, 20% of children with overactive bladder syndrome (OAB) fail to improve their bladder function. To approach the need for alternative strategies, an inpatient bladder rehabilitation ‘voiding school’ program was established.
Source: Journal of Pediatric Urology - Category: Urology & Nephrology Authors: Source Type: research
This study demonstrates positive effects of combination drug therapy on symptoms of OAB, possibly indicating it to be a good option for treatment of OAB during concomitant cystitis. It remains to be determined if increased release of NO is crucial for successful pharmacological treatment of bladder overactivity during cystitis.
Source: Pharmacology Research and Perspectives - Category: Drugs & Pharmacology Authors: Tags: ORIGINAL ARTICLE Source Type: research
The objective this study is to publish the study protocol that aims to investigate whether urgency decreases after treatment with both of the techniques.MethodsThis randomized controlled clinical trial will include 99 women, aged more than 18  years old, with urgency (score ≥ 8 in the Overactive Bladder-Validated 8-Question Awareness Tool [OAB-V8]). Women will be randomly allocated into three groups: TTNS, PTES, and placebo. The following questionnaires will be applied: the Anamnesis Record, the Incontinence Questionnaire Overact ive Bladder, the King’s Health Questionnaire, the 24-Hour Voidin...
Source: Trials - Category: Research Source Type: clinical trials
CONCLUSIONS: UDS can have a useful role in the diagnosis of idiopathic OAB in women given the possibility to gain a precise diagnosis and, therefore, a tailored treatment based on the underlying cause. The integration of clinics with UDS and all the other diagnostic available tools is desirable. PMID: 32026667 [PubMed - as supplied by publisher]
Source: Minerva Urologica e Nefrologica - Category: Urology & Nephrology Tags: Minerva Urol Nefrol Source Type: research
AbstractBackgroundSelective antimuscarinics may offer a favorable safety profile over non-selective antimuscarinics for the management of overactive bladder (OAB) in patients with dementia.ObjectiveTo test the hypothesis that non-selective antimuscarinics are associated with increased risk of mortality compared to selective antimuscarinics in older adults with dementia and OAB.DesignPropensity score-matched retrospective new-user cohort design among Medicare beneficiaries in community settings.PatientsOlder adults with dementia and OAB with incident antimuscarinic use.Main MeasuresThe primary exposure was antimuscarinic me...
Source: Journal of General Internal Medicine - Category: Internal Medicine Source Type: research
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