Detection and verification of 2.3 million cancer mutations in NCI60 cancer cell lines with a cloud search engine.

We present and examine the abilities of Bolt, a new cloud-based proteomics search engine to search for the presence of over 2.3 million known cancer mutations in a matter of minutes while still performing a standard proteomics search that includes 31 post translational modifications. We use previously published proteomics data sets and identify mutations that are verified using genomic studies as well as previous proteomics efforts. Our results also emphasize the need to search for mutations in a comprehensive manner while still searching for both common and rare PTMs. SIGNIFICANCE: We present and examine the abilities of Bolt, a new cloud-based proteomics search engine to search for the presence of over 2.3 million known cancer mutations in a matter of minutes while still performing a standard proteomics search that includes 31 post translational modifications. No other proteomics search software can do so.
PMID: 31445215 [PubMed - as supplied by publisher]

Source: Journal of Proteomics - August 20, 2019 Category: Biochemistry Authors: Prakash A, Majumder S, Ahmad S, Varkey M, Anish TA, Jenkins C, Rigby M, Orsburn B Tags: J Proteomics Source Type: research