A Method for Targeted Nonviral siRNA Delivery in Cancer and Inflammatory Diseases.

A Method for Targeted Nonviral siRNA Delivery in Cancer and Inflammatory Diseases. Methods Mol Biol. 2020;2059:155-166 Authors: Kandil R, Xie Y, Mehta A, Merkel O Abstract Small interfering RNA (siRNA)-based therapy has been subject of intense research since the discovery of RNA interference (RNAi), providing a tool to potentially silence any chosen gene. Nevertheless, efficient delivery still presents a major hurdle to translating this promising technology into medical practice. Here, we describe a straightforward method to prepare and characterize an effective delivery system consisting of low-molecular-weight polyethylenimine (PEI) and transferrin (Tf). Tf-PEI polyplexes are not only able to successfully transport and protect the sensitive nucleic acid payload from degradation but also to selectively deliver the siRNA to transferrin receptor (TfR)-overexpressing cells, playing key roles in the pathology of numerous cancer types as well as inflammatory diseases. PMID: 31435920 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research