Identification of new components of the RipC-FtsEX cell separation pathway of Corynebacterineae
by Hoong Chuin Lim, Joel W. Sher, Frances P. Rodriguez-Rivera, Coralie Fumeaux, Carolyn R. Bertozzi, Thomas G. Bernhardt Several important human pathogens are represented in the Corynebacterineae suborder, includingMycobacterium tuberculosis andCorynebacterium diphtheriae. These bacteria are surrounded by a multilayered cell envelope composed of a cytoplasmic membrane, a peptidoglycan (PG) cell wall, a second polysaccharide layer called the arabinogalactan (AG), and finally an outer membrane-like layer made of mycolic acids. Several anti-tuberculosis drugs target the biogenesis of this complex envelope, but their efficacy is declining due to resistance. New therapies are therefore needed to treat diseases caused by these organisms, and a better understanding of the mechanisms of envelope assembly should aid in their discovery. To this end, we generated the first high-density library of transposon insertion mutants in the model organismC.glutamicum. Transposon-sequencing was then used to define its essential gene set and identify loci that, when inactivated, confer hypersensitivity to ethambutol (EMB), a drug that targets AG biogenesis. Among the EMBs loci were genes encoding RipC and the FtsEX complex, a PG cleaving enzyme required for proper cell division and its predicted regulator, respectively. Inactivation of the conservedsteAB genes (cgp_1603 –1604) was also found to confer EMB hypersensitivity and cell division defects. A combination of quantitative microscopy, ...
Publication date: 17 September 2019Source: Cell Reports, Volume 28, Issue 12Author(s): Krishna C. Chinta, Md. Aejazur Rahman, Vikram Saini, Joel N. Glasgow, Vineel P. Reddy, Jeremie M. Lever, Shepherd Nhamoyebonde, Alasdair Leslie, Ryan M. Wells, Amie Traylor, Rajhmun Madansein, Gene P. Siegal, Veena B. Antony, Jessy Deshane, Gordon Wells, Kievershen Nargan, James F. George, Pratistadevi K. Ramdial, Anupam Agarwal, Adrie J.C. Steyn
ConclusionTaken together, these results suggested that inhibition of the PD-1/PD-L1 signaling pathway enhanced the innate immune response of alveolar macrophages to Mycobacterium TB in mice.
Condition: Tuberculosis Interventions: Other: ACF with a seed-and-recruit model; Other: ACF targeting household and neighborhood contacts; Other: ACF targeting the older population using mobile screening units; Other: Passive case finding Sponsors: National University, Singapore; KHANA Center for Population Health Research; National Center for Tuberculosis and Leprosy Control; Cambodian Anti-Tuberculosis Association Not yet recruiting
Conclusions Stroke involving the basal ganglia best differentiates CNSTB patients who are HIV+ from those HIV-. This finding was not correlated with meningeal enhancement suggesting that small arteries involvement might precede it. Therefore, we think that HIV+ patients with a new onset of stroke should be evaluated for CNSTB. Follow-up MRI should also be planned since meningeal enhancement might appear in later stages of the disease.
Publication date: October 2019Source: The Lancet Global Health, Volume 7, Issue 10Author(s): Juan F Vesga, Nimalan Arinaminpathy
Publication date: October 2019Source: The Lancet Global Health, Volume 7, Issue 10Author(s): Christian Wejse, Romain Ragonnet, Emma S McBryde, James M Trauer
In conclusion, IFX did not exacerbate production of inflammatory cytokines, and did not affect expression of TNFR, proliferation of ARPE-19, HTLV-1 proviral load, or apoptosis of ARPE-19. These results suggest that IFX does not exacerbate HTLV-1-related inflammation in the eye and represents an acceptable treatment option under HTLV-1 infectious conditions.
Tuberculosis (TB), caused by the human pathogens Mycobacterium tuberculosis (Mtb) and Mycobacterium africanum, has plagued humanity for millennia and remains the deadliest infectious disease in the modern world. Mycobacterium tuberculosis and M. africanum can be subdivided phylogenetically into seven lineages exhibiting a low but significant degree of genomic diversity and preferential geographic distributions. Human genetic variability impacts all stages of TB pathogenesis ranging from susceptibility to infection with Mtb, progression of infection to disease, and the development of distinct clinical subtypes. The genetic ...
ConclusionOur study showed the urgent need of a better veterinary control with the implementation of effective and comprehensive strategies in order to reach a good protection of animals as well as human health.
[Monitor] Budaka District leaders have expressed concern over the rising cases of tuberculosis disease (TB).