Mesenchymal stem cells improve the healing of burn wounds: A pilot safety study of the administration of mesenchymal stem cells in the treatment of burn wounds

Introduction: Options for therapy of severe burns are limited and generally include local wound care, debridement, grafting, and long-term care. Mesenchymal stem cells (MSCs) have been used in applications directed at the repair and regeneration of damaged tissue. There is little known regarding the clinical application of allogenic MSCs in the treatment of second degree burn injury. This pilot study establishes the safety of MSC therapy for burn wounds in two dose escalations, in addition to providing both observational and objective data that this treatment protocol improves the healing of these injuries.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Source Type: research

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Abstract Burns are a global public health issue of great concern. The formation of scars after burns and physical dysfunction of patients remain major challenges in the treatment of scars. Regenerative medicine based on cell therapy has become a hot topic in this century. Adipose-derived stem cells (ADSCs) play an important role in cell therapy and have become a promising source of regenerative medicine and wound repair transplantation. However, the anti-scarring mechanism of ADSCs is still unclear yet. With the widespread application of ADSCs in medical, we firmly believe that it will bring great benefits to pati...
Source: Current Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Curr Stem Cell Res Ther Source Type: research
Thermal injury leads to loss of the regenerative capacity of the skin and scarring becomes a significant risk, due to the inability to emulate normal skin architecture and adnexal structures. Mesenchymal stem cells (MSC) have been used in a variety of clinical applications to repair and regenerate damaged tissue. MSC ’s can stimulate cellular processes such as fibroblast proliferation, migration, and endothelial angiogenesis, as well as modulate the host’s immune response to favor more optimal wound healing.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Source Type: research
We describe an isolation method, characterize a population, and investigate its potential for cell banking and thus suitability as a potential product for cell grafting therapies. Our results show hFDF and a bone marrow-derived mesenchymal stem cell (BM-MSC) line shared identification markers and in vitro multilineage differentiation potential into osteogenic, chondrogenic, and adipogenic lineages. The hFDF population exhibited similar cell characteristics as BM-MSC while producing lower pro-inflammatory cytokine IL-6 levels and higher levels of the wound healing factor hepatocyte growth factor. We demonstrate in vitro dif...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research
ConclusionThe effect of MAPC secretome on the capacity of keratinocytes, fibroblast and endothelial cells to migrate and proliferate was determined in vitro using scratch wound closure and WST1 assay respectively. Secretome-treated fibroblasts were also immunostained for collagen 1 and 3 to investigate its effect on matrix production. Additionally, second degree burns were created on the dorsal surface of mice (n=8/group) and 5 × 105 MAPCs in 100µl PBS were administered via intradermal injection to the wound margins, 24h post-burn injury. The burns were imaged daily until day 7, for macroscopic wound area d...
Source: Cytotherapy - Category: Cytology Source Type: research
In this study, a combination of hylauronic acid (HA) and ASCs (HA/ASCs) was applied on burn wounds and the injured area was then covered by an ADM dressing in a rat model (ADM‐HA/ASCs). Wound healing was evaluated by histopathological, histomorphometrical, molecular, biochemical, and scanning electron microscopy assessments on days 7, 14, and 28 post‐wounding. ADM‐HA/ASCs stimulated healing significantly more than the ADM‐HA and ADM treated wounds, as it led to reduced inflammation, and improved angiogenesis and enhanced granulation tissue formation. Expression of interleukin‐1β (IL‐1β) and transformi...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research
Conclusion Massive SI and immune cell paralysis associated with ACLF represent the extreme severity of CAID in response to an infectious or sterile challenge. The severe immune disturbance plays a pivotal role in the pathogenesis of the distinctive features of ACLF: organ failure and bacterial infection susceptibility. Excessive SI in ACLF results from the massive activation and dysfunction of an innate immune system challenged by increased PAMPs and DAMPs. SI leads to cell and tissue immunopathology contributing to hepatic and extrahepatic organ failure. Concomitantly, the course of ACLF is associated with a disproportio...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In this study, we report the age-associated differences between fetal MSC (fMSC) populations and MSCs isolated from elderly donors with respect to their transcriptomes. We successfully reprogrammed fMSCs (55 days post conception) and adult MSC (aMSC; 60-74 years) to iPSCs and, subsequently, generated the corresponding iMSCs. In addition, iMSCs were also derived from ESCs. The iMSCs were similar although not identical to primary MSCs. We unraveled a putative rejuvenation and aging gene expression signature. We show that iMSCs irrespective of donor age and cell type re-acquired a similar secretome to that of th...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
This reporter group also includes lines driven by ubiquitous promoters like CMV and human ubiquitin C. These are particularly useful for cut-and-paste, transplantation-based experiments to label and fate map regions of host embryos. The second group of reporter expression lines includes transgenics marking subcellular organelles (Supplementary Table S1B). These are highly useful for the study of molecular processes involved in cell function and are especially effective when utilized in the context of Xenopus egg extracts, the only cell-free system that permits full investigation of all DNA transactions related to cell cyc...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
In this study, we firstly tested the effects of QJSB on leucopenia using mice induced by cyclophosphamide. Our results suggested that QJSB significantly raised the number of peripheral white blood cells, platelets and nucleated bone marrow cells. Additionally, it markedly enhanced the cell viability and promoted the colony formation of bone marrow mononuclear cells. Furthermore, it reversed the serum cytokines IL-6 and G-CSF disorders. Then, using transcriptomics datasets and metabonomic datasets, we integrated transcriptomics-based network pharmacology and metabolomics technologies to investigate the mechanism of action o...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Expression of the STAT3 gene influences a number of vital cellular processes, such as mitochondrial activity, cellular differentiation, and cellular proliferation. Researchers have investigated its activity in the context of spurring greater regenerative activity in heart muscles, for example. Arguably this is a good example of a regulatory gene that is involved in too many processes to make it a good target for therapeutics, however. More specific, lower-level mechanisms for specific desired goals would be helpful. That requires slow and costly investigative work, however, picking apart the relationships between proteins ...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
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