Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis.

Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis. Methods Mol Biol. 2019;1930:25-32 Authors: Ng AS, Ong ST, Kelleher D, Verma NK Abstract The exploration screening of phenotypic changes in motile T-cells within a signaling environment has always been an arduous task due to the sheer population of these microscopic cells. In recent years, High-Content Analysis (HCA) has gained epochal momentum and has allowed for a wider range of quantitative multiplexed cell-based assays in the field of lymphocyte signaling. In this chapter, we consolidate our understanding and describe the technical approach and methodology to quantify T-cell migratory phenotypes using HCA. Optimizations to be adopted to generate high-quality cytological images of motile T-cells and subsequent analysis using HCA are detailed as well. PMID: 30610595 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research