LC-MS-based metabolomics analysis to identify meprin β-associated changes in kidney tissue from mice with STZ-induced type 1 diabetes and diabetic kidney injury.

LC-MS-based metabolomics analysis to identify meprin β-associated changes in kidney tissue from mice with STZ-induced type 1 diabetes and diabetic kidney injury. Am J Physiol Renal Physiol. 2019 Aug 14;: Authors: Gooding J, Cao L, Ahmed F, Mwiza JM, Fernander M, Whitaker C, Acuff Z, McRitchie S, Sumner S, Ongeri EM Abstract Meprin metalloproteases have been implicated in the pathophysiology of diabetic kidney disease (DKD). SNPs in the meprin β gene associated with DKD in the Pima Indians, a Native American ethnic group with extremely high prevalence of DKD. In diabetic African American men, urinary meprin excretion positively correlated with severity of kidney injury. In mice, meprin activity decreased at the onset of diabetic kidney injury. Several studies have identified meprin targets in the kidney. However, it's not known how proteolytic processing of the targets by meprins impacts the metabolite milieu in kidneys. In the current study, global metabolomics analysis identified differentiating metabolites in kidney tissues from wild-type (WT) and meprin β knockout (βKO) mice with STZ-induced type 1 diabetes. Kidney tissues were harvested at 8 weeks post-STZ and analyzed by a hydrophilic interaction liquid chromatography (HILIC) ultra-performance liquid chromatography-quadrupole time-of flight mass spectrometry (UPLC-QTOF MS). Principal components analysis identified more than 200 peaks associated with diabetes. Meprin expressi...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research