Measuring redox effects on the activities of intracellular proteases such as the 20S Proteasome and the Immuno-Proteasome with fluorogenic peptides.

In this study we analyze different parameters, such as sample concentration, AMC conjugated substrate concentration, duration of assay, and frequency of measurements, and examine how they impact the determination of Proteasome and Immuno-Proteasome activities using fluorogenic peptide substrates.
PMID: 31351175 [PubMed - as supplied by publisher]

Source: Free Radical Biology and Medicine - July 23, 2019 Category: Biology Authors: Bonet-Costa V, Sun PY, Davies KJA Tags: Free Radic Biol Med Source Type: research