3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration.

3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration. Methods Mol Biol. 2019;1953:151-162 Authors: Osswald A, Hedrich V, Sommergruber W Abstract The cross talk between tumor cells and other cells present in the tumor microenvironment such as stromal and immune cells highly influences the behavior and progression of disease. Understanding the underlying mechanisms of interaction is a prerequisite to develop new treatment strategies and to prevent or at least reduce therapy failure in the future. Specific reactivation of the patient's immune system is one of the major goals today. However, standard two-dimensional (2D) cell culture techniques lack the necessary complexity to address related questions. Novel three-dimensional (3D) in vitro models-embedded in a matrix or encapsulated in alginate-recapitulate the in vivo situation much better. Cross talk between different cell types can be studied starting from co-cultures. As cancer immune modulation is becoming a major research topic, 3D in vitro models represent an important tool to address immune regulatory/modulatory questions for T, NK, and other cells of the immune system. The 3D systems consisting of tumor cells, fibroblasts, and immune cells (3D-3) already proved as a reliable tool for us. For instance, we made use of those models to study the molecular mechanisms of the cross talk of non-small cell lung cancer (NSCLC) and fibroblasts, to unveil macrophage plasticity in the tu...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

Related Links:

This study examined the incidence of pathologic N2 non-small-cell lung cancer (NSCLC) and prognostic factors affecting survival in these patients. Methods: A total of 119 patients who underwent surgery for NSCLC (lobectomy, pneumonectomy) between January 2008 and December 2016 were evaluated retrospectively. The patients with pN2 included in the study were assessed in 2 groups; single pN2 and multiple pN2. Results: The most common type of resection was lobectomy (56.3%). Ninety-four patients (79%) received adjuvant therapy. Eighty-six patients (72.3%) had single-station pN2 and 33 (27.7%) had multiple pN2. The 5-year survi...
Source: Acta Chirurgica Belgica - Category: Surgery Tags: Acta Chir Belg Source Type: research
ConclusionThe cellular effects produced by SFN in NSCLC cells are largely mediated by SFN-induced production of ROS. Cells with higher levels of EGFR were more resistant to SFN treatment and showed resistance to SFN-induced apoptosis, suggesting that high EGFR levels protect cells from SFN-induced apoptosis. Despite this, we found that SFN retained the ability to inhibit the growth of NSCLC tumors with high-level EGFR expression in vivo.Graphical abstractModel for the effects of different EGFR expression in SFN-mediated cellular effects.
Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
We examined the trends in survival based on treatment modality among non-small cell lung cancer (NSCLC) patients in the province of Ontario, Canada, from 2007 to 2015. Methods: We investigated the trends in survival based on treatment modality. Results: Among 56,417 identified patients, the mean age at diagnosis was 70.1 years (SD = 10.7). Treatment modalities varied significantly over time (p
Source: Cancer Investigation - Category: Cancer & Oncology Tags: Cancer Invest Source Type: research
ConclusionsEven when whole tumor uptakes in patients with T3/T4 tumors markedly decreases after induction chemoradiotherapy, surgical treatment is still be indicated because of possible residual tumors. Tumor remission at extrapulmonary involvement sites could be predicted by extrapulmonary uptake values. Both whole- and extrapulmonary uptake values after induction chemoradiotherapy could be used to predict prognosis.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsOpioid dependence in the over 65-year old population after lung resection is high but was significantly lower among those who received minimally invasive surgery, in addition to other factors.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
The spine is a common site of metastasis from non-small cell lung cancer (NSCLC), a disease hallmarked by heterogeneity in molecular alterations. Traditional spine radiation treatment (RT) with conventionally fractionated external beam radiation therapy (cEBRT) is associated with high local failure (LF) rates in patients surviving more than 12 months. Treatment with advanced techniques including stereotactic body radiation therapy (SBRT) significantly reduces LF. We hypothesized that specific genomic mutations in NSCLC metastases impart increased risk of LF, and that treatment with SBRT would prevent LF in metastases harbo...
Source: The Spine Journal - Category: Orthopaedics Authors: Source Type: research
In this study, we developed dequalinium (DQA) modified paclitaxel plus ligustrazine micelles to destroy vasculogenic mimicry (VM) channels and inhibit tumour metastasis. In vitro assays showed that the targeting micelles with centralized particle size distribution showed not only vigoroso cytotoxicity on A549 cells but also strong inhibition on VM channels and tumour metastasis. Mechanism studies indicated that the DQA modified paclitaxel plus ligustrazine micelles could down-regulate the expressions of VEGF, MMP2, TGF-β1 and E-cadherin in A549 cells. In vivo assays indicated that the targeting drug-loaded micelles co...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Source: OncoTargets and Therapy - Category: Cancer & Oncology Tags: OncoTargets and Therapy Source Type: research
Authors: Zhang L, Tao X, Fu Q, Ge C, Li R, Li Z, Zhu Y, Tian H, Li Q, Liu M, Hu H, Zeng B, Lin Z, Li C, Luo R, Song X Abstract Despite the increasing number of available therapeutic methods, the prognosis of non‑small cell lung cancer (NSCLC) remains poor. Furthermore, side effects are an important limiting factor in the treatment of NSCLC. Therefore, developing an efficacious, safe, affordable and easily accessible chemotherapeutic agent is necessary for NSCLC treatment. As a natural chemical produced by Zingiberaceae plants, curcumin exerts distinct antitumor effects on several tumor types. In the present study...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
MicroRNA‑208a directly targets Src kinase signaling inhibitor 1 to facilitate cell proliferation and invasion in non‑small cell lung cancer. Mol Med Rep. 2019 Jul 31;: Authors: Liu L, Wang W, Gao S, Wang X Abstract The abnormal expression of microRNAs (miRNAs/miRs) has a critical function in the formation and progression of non‑small cell lung cancer (NSCLC). Therefore, understanding the association between NSCLC and dysregulated miRNAs may allow for the identification of novel diagnostic and therapeutic biomarkers for patients with this malignancy. Previous studies have validated miR‑208a as a...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
More News: Cancer | Cancer & Oncology | Lung Cancer | Molecular Biology | Non-Small Cell Lung Cancer | Study