The relationship between DJ-1 and S100A8 in human primary alveolar type II cells in emphysema.

The relationship between DJ-1 and S100A8 in human primary alveolar type II cells in emphysema. Am J Physiol Lung Cell Mol Physiol. 2019 Jul 17;: Authors: Lin CR, Bahmed K, Tomar D, Marchetti N, Criner GJ, Bolla S, Wilson MA, Madesh M, Kosmider B Abstract Pulmonary emphysema is characterized by alveolar type II (ATII) cell death, destruction of alveolar wall septa, and irreversible airflow limitation. Cigarette smoke induces oxidative stress and is the main risk factor for this disease development. ATII cells isolated from non-smokers, smokers, and patients with emphysema were used for this study. High apoptosis was detected in ATII cells obtained from individuals with this disease. DJ-1 and S100A8 have cytoprotective functions against oxidative stress-induced cell injury. Reduced DJ-1 and S100A8 interaction was found in ATII cells in patients with emphysema by immunoprecipitation and Western blotting. The molecular function of S100A8 was determined by an analysis of the oxidation status of its cysteine residues using chemoselective probes. Decreased S100A8 sulfination was observed in emphysema patients. In addition, its lower levels correlated with higher cell apoptosis induced by cigarette smoke extract in vitro. Cysteine at position 106 within DJ-1 is a central redox-sensitive residue. DJ-1 C106A mutant construct abolished the cytoprotective activity of DJ-1 against cell injury induced by cigarette smoke extract. Furthermore, a mol...
Source: Am J Physiol Lung Ce... - Category: Respiratory Medicine Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research