Production of High-Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells.

Production of High-Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells.
Methods Mol Biol. 2019;1940:47-61
Authors: Larcombe MR, Manent J, Chen J, Mishra K, Liu X, Nefzger CM
Abstract
Lentiviral gene transfer technologies exploit the natural efficiency of viral transduction to integrate exogenous genes into mammalian cells. This provides a simple research tool for inducing transgene expression or endogenous gene knockdown in both dividing and nondividing cells. This chapter describes an improved protocol for polyethylenimine (PEI)-mediated multi-plasmid transfection and polyethylene glycol (PEG) precipitation to generate and concentrate lentiviral vectors.
PMID: 30788817 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - July 13, 2019 Category: Molecular Biology Authors: Larcombe MR, Manent J, Chen J, Mishra K, Liu X, Nefzger CM Tags: Methods Mol Biol Source Type: research