Overexpression of PGE2 synthase by in vivo transient expression enhances immunocompetency along with fitness cost in a lepidopteran insect [RESEARCH ARTICLE]

This study used in vivo transient expression (IVTE) technique to induce overexpression and assessed subsequent physiological alteration in S. exigua. Se-PGES2 was cloned into a eukaryotic expression vector and transfected to Sf9 cells to monitor its heterologous expression. The Sf9 cells expressed the recombinant Se-PGES2 (rSe-PGES2) at an expected size (~47 kDa), which was localized in cytoplasm. The recombinant expression vector was then used to transfect larvae of S. exigua. Hemocytes collected from the larvae treated with IVTE expressed rSe-PGES2 gene for at least 48 h. The larvae treated with IVTE exhibited an enhanced competency in cellular immune response measured by hemocyte nodule formation. In addition, IVTE treatment of Se-PGES2 induced gene expression of antimicrobial peptides without any immune challenge. The larvae treated with IVTE became significantly resistant to infection of an entomopathogenic nematode, Steinernema monticolum or to infection to its symbiotic bacterium, Xenorhabdus hominickii. However, IVTE-treated S. exigua larvae suffered from reduced pupal size and fecundity.

Source: Journal of Experimental Biology - July 4, 2019 Category: Biology Authors: Ahmed, S., Hasan, M. A., Kim, Y. Tags: RESEARCH ARTICLE Source Type: research