Simultaneous Separation and Quantitation of Three Phytosterols from the Sweet Potato, and Determination of their Anti-Breast Cancer Activity

Publication date: Available online 2 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Pu Jiang, Bing Han, Lingmin Jiang, Yuanfeng Li, Yang Yu, Heshan Xu, Zhaoxing Li, Deqi Zhou, Xiaoxin Jia, Xuegang Li, Xiaoli YeAbstractThe present study provides the method for simultaneous separation and determination of concentration and evaluates anti-breast cancer activity of three phytosterols from the sweet potato (Ipomoea batatas L.): daucosterol linolenate (DLA), daucosterol linoleate (DL), and daucosterol palmitate (DP). A cell viability assay revealed that the three phytosterols had a stronger inhibitory effect on MCF-7 than MDA-MB-231 breast cancer cell line, and had no effects on non-tumorigenic MCF-10A cells. In vivo experiments demonstrated that DLA, DL, and DP suppressed tumor growth in MCF-7 xenograft breast cancer model in nude mice. Given the anti-breast cancer activity of DLA, DL, and DP, an HPLC method for the determination of their content in the sweet potato was developed. The method had satisfactory linearity (R2 = 0.9992–0.9999). The limits of detection (LOD) were in the range of 2.5–10 μg/mL, the limits of quantification (LOQ) were 5–25 μg/mL, and the recovery rates were 97.64–103.02%. Additionally, the HPLC method was successfully validated in eight sweet potato cultivars. This novel technique can be applied for the determination of DLA, DL, and DP in the sweet potato.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research