Can trophectoderm RNA analysis predict human blastocyst competency?
Can trophectoderm RNA analysis predict human blastocyst competency? Syst Biol Reprod Med. 2019 Jun 27;:1-14 Authors: Ntostis P, Kokkali G, Iles D, Huntriss J, Tzetis M, Picton H, Pantos K, Miller D Abstract A systematic review of the literature showed that trophectoderm biopsy could assist in the selection of healthy embryos for uterine transfer without affecting implantation rates. However, previous studies attempting to establish the relationship between trophectoderm gene expression profiles and implantation competency using either microarrays or RNA sequencing strategies, were not sufficiently optimized to handle the exceptionally low RNA inputs available from biopsied material. In this pilot study, we report that differential gene expression in human trophectoderm biopsies assayed by an ultra-sensitive next generation RNA sequencing strategy could predict blastocyst implantation competence. RNA expression profiles from isolated human trophectoderm cells were analysed with established clinical pregnancy being the primary endpoint. Following RNA sequencing, a total of 47 transcripts were found to be significantly differentially expressed between the trophectoderm cells from successfully implanted (competent) versus unsuccessful (incompetent) blastocysts. Of these, 36 transcripts were significantly down-regulated in the incompetent blastocysts, including Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1) and Cytochrome P450 Family 11 Subfamily A Member 1...
[African Arguments] The ECOWAS court ruling could have important implications for Sierra Leone, external partners and other countries with similar bans.
ConclusionFor elective blastocyst stage transfer and a freezing strategy performed according to the Taiwan National Assisted Reproduction Act, a young female age ≤ 35 with positive pregnancy status due to the original IVF treatment, the production of ≥ 6 embryos, and the cryopreservation of ≥ 2 blastocysts may increase the likelihood of abandoning embryos in the future.
Conclusions: In our study, the women who followed the water-based exercise program gained less weight during pregnancy, which facilitated a better rate of spontaneous, non-instrumental childbirth, together with a better Apgar test score at five minutes.
CONCLUSIONSHealth care providers and facilities should provide transmasculine people with tailored contraceptive information and care that address their specific gender ‐affirmation needs and contraceptive preferences in safe, inclusive and supportive clinical settings.
ConclusionIn conclusion, transvaginal ultrasound guided local and systemic methotrexate injection may be performed successfully for the cases of advanced gestational age with fetal cardiac activity and high serum β-hCG levels.
ConclusionOur results suggest that maternal APOL1 G1 risk allele may contribute to the development of early-onset PE in South African pregnant women of African ancestry either directly or by transmission of a APOL1 risk allele to the foetus.
[263Chat] Zimbabwe's under-performing economy has resulted in countless societal problems chief among them moral decadence which results in under-aged children falling pregnant and seeking abortion services from illegal backyard facilities, 263Chat's visit to Harare's Epworth suburb has revealed.
Healthcare professionals from the University of Pittsburgh Medical Center are addressing maternal morbidity by focusing on mothers' health in their "fourth trimester," the end of the pregnancy through the first year. Describes that there is a higher risk for maternal health issues in rural areas and explains how communities can help.
Authors: Fournier E Abstract Carpal tunnel syndrome (CTS) is too common a condition not to daily interact with the practitioner, if only because of its entanglement to other pathologies, causal or chance association. The typical symptomatology, with hand paresthesia and morning pain upon waking, is related to a median nerve injury in the confined space of the carpal tunnel, more often by local inflammation and tenosynovitis of the finger flexors (repetitive activity of the hands). SCC may be secondary to situations (pregnancy) or conditions (edema, hypothyroidism…), which exaggerate the ordinary pathophysiol...
In Reply We found in a double-blind, randomized, placebo-controlled trial that high-dose vitamin D supplementation during pregnancy was associated with reduced risk of recurrent wheeze and enamel defects in offspring. Deng and Niu suggest that this finding was flawed by residual confounding. However, the design mitigates confounding. This is demonstrated in our study showing equal distribution of lifestyle-related factors between groups. Therefore, our results cannot be biased from residual confounding.