Mitochondrial Dynamics and Activity in Legionella-Infected Cells.

Mitochondrial Dynamics and Activity in Legionella-Infected Cells. Methods Mol Biol. 2019;1921:205-220 Authors: Song OR, Brodin P, Buchrieser C, Escoll P Abstract The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages. PMID: 30694494 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research