Real-time bacterial detection with an intracellular ROS sensing platform

Publication date: Available online 19 June 2019Source: Biosensors and BioelectronicsAuthor(s): J.M. Hicks, R. Halkerston, N. Silman, S.K. Jackson, J.W. Aylott, F.J. RawsonAbstractReactive oxygen species are highly reactive molecules that as well as being ubiquitously expressed throughout the body, are also known to be involved in many diseases and disorders including bacterial infection. Current technology has limited success in the accurate detection and identification of specific reactive oxygen species. To combat this, we have developed an electrochemical biosensor that is constructed from single walled carbon nanotubes that have been immobilised on an indium tin oxide surface functionalised with osmium-based compound. This sensor was integrated within mouse macrophage cells (RAW 264.7) with multiple serotypes of bacteria used to initiate an immune response. Intracellular hydrogen peroxide was then measured in response to the interaction of the lipopolysaccharides, present on the outer wall of Gram-negative bacteria, with the Toll-like Receptor 4. Additional controls of n-acetylcysteine and sodium pyruvate were implemented to prove the specificity of the sensor towards hydrogen peroxide. The sensors were found to have a lower limit of detection of 368 nM hydrogen peroxide. An increase in intracellular hydrogen peroxide was detected within 3 seconds of interaction of the bacteria with the macrophage cells. This low limit of detection combined with the rapid response of th...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research

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Publication date: Available online 15 July 2019Source: The Journal of Allergy and Clinical Immunology: In PracticeAuthor(s): Lisa M. Bartnikas, Michelle F. Huffaker, William J. Sheehan, Watcharoot Kanchongkittiphon, Carter R. Petty, Robert Leibowitz, Michael C. Young, Wanda Phipatanakul
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
Publication date: Available online 15 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Emad I. Wafa, Sean M. Geary, Kathleen A. Ross, Jonathan T. Goodman, Balaji Narasimhan, Aliasger K. SalemAbstractThe primary objective of this study was to enhance the antitumor efficacy of a model cancer vaccine through co-delivery of pentaerythritol lipid A (PELA), an immunological adjuvant, and a model tumor antigen, ovalbumin (OVA), separately loaded into polyanhydride particles (PA). In vitro experiments showed that encapsulation of PELA into PA (PA-PELA) significantly enhanced its stimulatory capacity on...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
Publication date: November 2019Source: Materials Science and Engineering: C, Volume 104Author(s): T. Arun, Suresh K. Verma, Pritam Kumar Panda, R. Justin Joseyphus, Ealisha Jha, Ali Akbari-Fakhrabadi, Pranesh Sengupta, D.K. Ray, V.S. Benitha, K. Jeyasubramanyan, P.V. SatyamAbstractNanomaterial based paints are in current demand in the area of surface protective coatings due to the significant advances made to improve their antibacterial and anticorrosion characteristics. In this work, we have developed magnetic graphene oxide (MGO) paint with the incorporation of cobalt ferrite (CF) and graphene oxide (GO) along with paint...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
In this study we present the first crystal structure model for bone apatite based on the analysis of individual nanocrystals by high resolution transmission electron microscopy (HRTEM). Crystallographic image processing of the obtained HRTEM images from different projections indicates symmetry reduction with respect to P63/m stoichiometric apatites and the presence of threefold symmetry along the c axis. Based on HRTEM observations and the measured Ca/P = 2 ratio we propose a structural model with phosphate-to-carbonate substitution and O vacancies localized along c axis, which explains the observed loss of 63 screw ax...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 14 July 2019Source: Materials Science and Engineering: AAuthor(s): Jin Zhang, Zhide Li, Fushun Xu, Cheng HuangAbstractRegulating the deformation and property synchronously is a key point of creep aging process. And pre-deformation has been considered to be a good regulating method. In this paper, the laws of the influence of pre-stretching degree on creep strain, strength property, precipitate during creep aging process of Al–Cu–Li alloy were studied, and the pre-stretching design methods suitable for creep aging process were explored. The results indicate that the introductio...
Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
Publication date: Available online 14 July 2019Source: Materials Science and Engineering: AAuthor(s): Xianwei Ren, Yuanchun Huang, Wenlong Zhou, Likai Zhao, Qiang Wang, Xudong MinAbstractPre-recovery (PR) treatment and the subsequent recrystallization of twin-roll cast (TRC) AA6016 (Al-1.04Si-0.5Mg-0.4Fe-0.16Mn) sheet are performed. The effects of PR on the recrystallization behavior, grain structure and mechanical properties are investigated. Results reveal that the refinement and homogeneity of the recrystallization grain structure first increase and then decrease when the PR temperature (PRT) is increased. This attribut...
Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
Publication date: Available online 14 July 2019Source: Materials Science and Engineering: AAuthor(s): Jing Wu, Chong Li, Yongchang Liu, Xingchuan Xia, Yuting Wu, Yefan Li, Haipeng WangAbstractIn present work, the unique envelope structure (about 1.1 μm in width, and ∼3 vol%) in an as-cast multiphase Ni3Al-based intermetallic alloy was characterized in detail, its formation mechanism during solidification and widening behavior during long-term aging process were analyzed, and relevant effects on creep behaviors of this Ni3Al-based intermetallic alloy under 800 oC/220 MPa were evaluated. Results show that the...
Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
Publication date: Available online 14 July 2019Source: Materials Research BulletinAuthor(s): Gohil S Thakur, Beluvalli E. Prasad, Thomas Doert, Yan Sun, Claudia Felser, Martin JansenAbstractReinvestigating a phase reported for the Na-Au-Bi-O system has revealed that both former structure determinations were incorrect and that the true composition is Na2Bi5AuO11. Its crystal structure (space group Pnma; a = 12.1629(8) Å, b = 7.7086(5) Å and c = 12.1696(8) Å) is related to orthorhombic NaBi2RuO6. Band structure calculations predict a topological trivial insulating s...
Source: Materials Research Bulletin - Category: Materials Science Source Type: research
Publication date: 1 November 2019Source: Materials Letters, Volume 254Author(s): S. Lanfredi, F.R. Praxedes, F.F. Ivashita, A. Paesano, M.A.L. NobreAbstractSuppression of the phase-transition type ferro-paraelectric from doping of KSr2Nb5O15 host-structure was investigated by set of techniques X-ray diffraction, Mössbauer spectroscopy and Dielectric spectroscopy at high temperature. High crystalline and single phase iron-doped niobate ceramic with Tetragonal Tungsten Bronze TTB-type structure, stoichiometry KSr2(FeNb4)O15-δ, was prepared by conventional technique using high energy milling. Both techniques Rietve...
Source: Materials Letters - Category: Materials Science Source Type: research
Publication date: 1 November 2019Source: Materials Letters, Volume 254Author(s): Xiao-Hua Huang, Meng Xu, Zhi-Xue Xu, Jian-Min Yan, Ren-Kui ZhengAbstractThe electric-field control of resistance change (ΔR/R) and magnetic properties has been investigated in the LaMnO3(LMO)/0.71Pb(Mg1/3Nb2/3)O3-0.29PbTiO3 (PMN-PT)(0 1 1) heterostructure. When the unipolar electric field (E) increases beyond the coercive field of PMN-PT, the ΔR/R vs E curve transforms from a loop-like behavior to a butterfly-like one. The electric-field-induced non-linear strain effect in the PMN-PT substrate realizes the modulation between lo...
Source: Materials Letters - Category: Materials Science Source Type: research
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