Development of DNA barcode for rapid identification of Epimedium elatum (Morren & Decne) from Northwestern Himalayas in India

Publication date: Available online 15 June 2019Source: Journal of Applied Research on Medicinal and Aromatic PlantsAuthor(s): Sajad A. Lone, Qazi P. Hassan, Suphla GuptaAbstractEpimedium elatum (Morren & Decne) is a rare monotypic high altitude medicinal species, endemic to shady forests in Northwestern Himalayas, India. Its recent chemoprofiling has revealed high concentration of Epimedin B, C & Icariin, thus matching quality standards of Herba Epimedii. DNA barcode was hitherto unreported in E.elatum. Three plastid genes (matK, rbcL & trnH-psbA) and one nuclear region gene (ITS) were partially sequenced to develop unique barcode and also investigate nucleotide diversity and phylogenetic relationship among five geographically diverse accessions of E.elatum. We used the Kimura 2-parameter (K2P) evolutionary model to calculate basic sequence statistics like conserved, variable, parsimony informative and singleton sites, including pairwise genetic distance in MEGA X software. Besides, we employed a maximum likelihood phylogenetic tree method for estimating the evolutionary divergences. The present study revealed correct blast validation of E.elatum populations with other Epimedium species. A total of 18 new sequences were obtained and submitted to NCBI Genbank via BankIT submission tool (MH615782–MH615799). Highest variable (polymorphic) sites were found in matK (269) followed by ITS (115); rbcL (18) and least in trnH-psbA (16). The evolutionary sequence divergence varied fro...
Source: Journal of Applied Research on Medicinal and Aromatic Plants - Category: Complementary Medicine Source Type: research