Interleukin-10 controls the protective effects of circulating microparticles from septic shock patients on tissue-engineered vascular media

During sepsis, inflammation can be orchestrated by the interaction between circulating and vascular cells, that under activation, release microparticles. Recently, we reported that increased circulating microparticles in septic patients play a pivotal role in ex vivo vascular function suggesting that they are protective against vascular hyporeactivity. The present study was designed to investigate the effects of septic microparticles on the contractile response of tissue-engineered vascular media (TEVM). TEVMs composed of only a media were produced by tissue engineering from human arterial smooth muscle cells isolated from umbilical cord. TEVMs were incubated with microparticles isolated from 16 septic patients. Microparticles were extracted from whole blood of septic subjects. TEVMs were incubated for 24 h with microparticles and used for the study of vascular contraction, direct measurements of nitric and superoxide anion production by electron paramagnetic resonance and quantification of mRNA cytokine expressions. Septic microparticles increased contraction induced by histamine in TEVM. This effect was not associated with inflammation, neither linked to the activation of NF-κB pathway, nor to the increase of inducible nitric oxide synthase and cyclooxygenase-2 expressions. In contrast, mRNA expression of interleukin-10 was enhanced. Then, we investigated the effect of interleukin-10 on vascular hyporeactivity induced by lipopolysaccharide (LPS). Although interleukin...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research