Expression analysis of vitamin D receptor-associated lncRNAs in epileptic patients

AbstractVitamin D has been vastly acknowledged as a neuroactive steroid controlling neurodevelopment. As it exerts its functions through activation of vitamin D receptor (VDR), several studies have assessed the role of VDR in brain function. More recently, a number of long non-coding RNAs (lncRNAs) have been recognized that alter expression of VDR. In the current study, we evaluated expression of fourVDR-related lncRNAs (LINC00511,LINC00346,SNHG6 andSNHG16) in peripheral blood of 40 epileptic patients and 39 healthy subjects using quantitative real time PCR method. The relative expression levels ofSNHG16 andLINC00511 were higher in epileptic patients compared with healthy subjects. ForSNHG16, the difference was only significant between male patients and male controls, whileLINC00511 had the opposite pattern. The results of Quantile regression model showed significant associations betweenSNHG6 andSNHG16 expressions and gender (P values of 0.027 and 0.009 respectively). Significant correlations were detected between expression levels ofSNHG6 andSNHG16 (r  = 0.32,P = 0.004),SNHG6 andLINC00346 (r  = 0.37,P = 0.001),SNHG16 andLINC00346 (r  = 0.30,P = 0.007) as well asSNHG16 andLINC00511 (r  = 0.29,P = 0.009). Expression ofLINC00346 was inversely correlated with vitamin D levels only in male epileptic patients (r  = −0.58,P = 0.011). Expression ofSNHG6 was correlated with vitamin D levels in male controls but no other subgroups (r  = 0.51,PÂ...

Source: Metabolic Brain Disease - June 10, 2019 Category: Neurology Source Type: research