Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions.

We describe generation of mouse naïve PS cells using LIF and inhibitors of MEK and GSK3 kinases and of mouse primed PS cells using FGF2 and IWR1 compound which induces the stabilization of Axin proteins.
PMID: 31175643 [PubMed - in process]

Source: Mol Biol Cell - June 10, 2019 Category: Molecular Biology Authors: De Los Angeles A, Okamura D, Wu J Tags: Methods Mol Biol Source Type: research