Comparison of two different methods for tigecycline susceptibility testing in acinetobacter baumannii.

COMPARISON OF TWO DIFFERENT METHODS FOR TIGECYCLINE SUSCEPTIBILITY TESTING IN ACINETOBACTER BAUMANNII. Acta Clin Croat. 2018 Dec;57(4):618-623 Authors: Bedenić B, Cavrić G, Vranić-Ladavac M, Barišić N, Karčić N, Tot T, Presečki-Stanko A, Lukić-Grlić A, Frančula-Zaninović S, Sreter KB Abstract - Tigecycline susceptibility testing (TST) presents a tremendous challenge for clinical microbiologists. Previous studies have shown that the Epsilometer test (E-test) and Vitek 2 automated system significantly overestimate the minimum inhibitory concentrations for tigecycline resistance compared to the broth microdilution method (BMM). This leads to very major errors or false susceptibility (i.e. the isolate is called susceptible when it is actually resistant). The aim of this study was to compare E-test against BMM for TST in carbapenem-resistant and carbapenem-susceptible Acinetobacter (A.) baumannii and to analyze changes in tigecycline susceptibility between two time periods (2009-2012 and 2013-2014), with BMM as the gold standard. Using the EUCAST criteria, the rate of resistance to tigecycline for the OXA-23 MBL-positive, OXA-23 MBL-negative and carbapenemase-negative strains for BMM was 54.5% (6/11), 29.4% (5/17) and 2.7% (1/37), respectively; the OXA-24/40 and OXA-58 producing organisms did not exhibit any resistance. With E-test, all OXA-23 MBL-positive organisms (11/11), 23.5% (4/17) of OXA-23 MBL-negative, and 4.1% of OXA-24/40 (3/74) st...
Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research

Related Links:

Authors: Koyuncu Özyurt Ö, Özhak B, Öğünç D, Yıldız E, Çolak D, Günseren F, Öngüt G Abstract Infections with multidrug resistant gram-negative bacteria is a growing problem especially in health care settings. Colistin is one of the last resort antibiotics for such infections in which treatment options are limited. Increasing resistance to colistin is a global problem. Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) study groups have recommended the ISO-standard broth microdilution ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
Publication date: Available online 15 August 2019Source: Biochemical Engineering JournalAuthor(s): Li Xia, Xiaotong Luo, Yunfei Zhu, Xiang Zhang, Lixin LuoAbstractApplications of immobilization technology to wastewater treatment have achieved mixed results, that is, high degradation efficiency or good reusability. In this research, alginate was used as supporting material of immobilized beads to maintain high degradation efficiency. Applying CaCl2 freeze drying and acidic solutions led to the reusability of the beads. Ca-alginate beads can be reused for more than 21 cycles of repeated CaCl2 freeze drying and 16 cycles of t...
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research
ier Michael Wink Infections caused by multidrug-resistant bacteria are a global emerging problem. New antibiotics that rely on innovative modes of action are urgently needed. Ranalexin is a potent antimicrobial peptide (AMP) produced in the skin of the American bullfrog Rana catesbeiana. Despite strong antimicrobial activity against Gram-positive bacteria, ranalexin shows disadvantages such as poor pharmacokinetics. To tackle these problems, a ranalexin derivative consisting exclusively of d-amino acids (named danalexin) was synthesized and compared to the original ranalexin for its antimicrobial potential and its bi...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
This study aimed to isolate and characterize bacterial endophytes and their crude extracts fromC.  macowanii bulbs. Endophytes were isolated using validated surface sterilization techniques, followed by phenotypic and genotypic profiles of the isolates. Crude extracts were extracted from the endophytes using ethyl acetate, while methanol:dichloromethane (1:1) was used to obtain crude extracts from the bulbs. Antibacterial activity of crude extract from each endophyte was investigated against selected pathogenic strains using the broth microdilution method, and anticancer activity against U87MG glioblastoma and A549 lu...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Chaitra Shankar, Agila Kumari Pragasam, Balaji Veeraraghavan, Anushree AmladiIndian Journal of Medical Microbiology 2019 37(1):91-94 Tigecycline is a reserve antibiotic increasingly used for the treatment of multidrug-resistant bacteria, especially Klebsiella pneumoniae and Acinetobacter baumannii. At present, there are concerns regarding the testing and interpretation of tigecycline susceptibility to bugs such as K. pneumoniae and A. baumannii, which limit clinicians in appropriate usage. Use of appropriate method for testing such as broth microdilution is essential. In addition, tigecycline susceptibility testing is a c...
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research
;r SA Abstract Empiric antibiotic therapy with a betalactam is standard of care in febrile neutropenia (FN), and is given to prevent early death. The addition of vancomycin is recommended in certain circumstances but the quality of evidence is low, reflecting the lack of clinical data. In order to characterize the epidemiology of early death and shock in FN, we reviewed all episodes of FN from 2003 to 2017 at University Hospital, Federal University of Rio de Janeiro, and looked at factors associated with shock at first fever and early death (within 3 days from first fever) by univariate and multivariate analyses. ...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Abstract We evaluated the toxicity of surfactants against different predatory bacteria. Tests with Bdellovibrio bacteriovorus HD100 and SDS, an anionic surfactant, showed the predator was very sensitive; 0.02% SDS completely killed the predatory population (7-log loss;
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
Publication date: Available online 9 August 2019Source: Journal of Hospital InfectionAuthor(s): Cheng-Yi Fan, Wan-Ting Lee, Tzu-Chun Hsu, Cheng-Han Lee, Shiao-Pei Wang, Wen-Shan Chen, Chien-Hua Huang, Chien-Chang LeeSummaryBackgrounds: Healthcare-associated infections (HAI) caused by multidrug-resistant Gram-negative bacteria (MDRGNB) have increased prevalence in intensive care units (ICUs). A common strategy to prevent HAI is bathing patients with chlorhexidine gluconate (CHG). However, the effectiveness of CHG bathing against multidrug-resistant Acinetobacter baumannii (MDRAB) is still controversial.AimTo perform a syste...
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
The goal of this study was to isolate, screen, and characterize Arctic microbial isolates from Expedition Fjord, Axel Heiberg Island, Nunavut, Canada capable of inhibiting the growth of foodborne and clinically relevant pathogens. Arctic bacteria were isolated from twelve different high Arctic habitats pertaining to active layer permafrost soil, saline spring sediments, lake sediments, and endoliths. This was achieved using (1) the cryo-iPlate, an innovative in situ cultivation device within active layer permafrost soil and (2) bulk plating of Arctic samples by undergraduate students that applied standard culturing. To mit...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Acinetobacter | Croatia Health | General Medicine | Microbiology | Study