Immobilization and Performance of Penicillin G Acylase on Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO Nanocomposites.

Immobilization and Performance of Penicillin G Acylase on Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO Nanocomposites. J Microbiol Biotechnol. 2019 May 24;: Authors: Lv Z, Yu Q, Wang Z, Liu R Abstract Magnetic Ni0.7Co0.3Fe2O4 nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites, on which Penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and 45 °C while immobilized PGA's activities reached the peak at pH 7.5 and 45 °C. immobilized PGA had better thermal stability than Free PGA at range of 30-50 °C for different time intervals. The activity of free PGA would be 0 and that of immobilized PGA still retained some activities at 60 °C after 2 h. Vmax and Km of immobilized PGA were 1.55 mol·min-1 and 0.15 mol·L-1, respectively. Free PGA's Vmax and Km separately were 0.74 mol·min-1 and 0.028 mol·L-1. Immobilized PGA displayed more than 50% activity after 10 successive cycles. We approved that the immobilized PGA with magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites would become a novel example for the immobilization of else amidohydrolase. PMID: 31154745 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research