Snapshot of chikungunya could lead to drugs, vaccines for viral arthritis

(Washington University School of Medicine) A team at Washington University School of Medicine in St. Louis has snapped high-resolution pictures of chikungunya virus latched onto a protein found on the surface of cells in the joints. The structures, published May 9, 2019 in the journal Cell, shows in atomic-level detail how the virus and cell-surface protein fit together -- data that promises to accelerate efforts to design drugs and vaccines to prevent or treat arthritis caused by chikungunya or related viruses.
Source: EurekAlert! - Biology - Category: Biology Source Type: news

Related Links:

In this study, 48,750 small molecules were screened in silico for their ability to bind to the ADP-ribose binding site of the CHIKV nsP3 macro domain. From this in silico analysis, 12 molecules were selected for in vitro analysis using a CHIKV subgenomic replicon in Huh-7 cells. Cell viability and CHIKV replication were evaluated and molecules C5 and C13 demonstrated 53 and 66% inhibition of CHIKV replication, respectively. By using a CHIKV-Dual luciferase replicon contain two reporter genes, we also demonstrated that the treatment with either compounds are probably interfering in the early replication rather than after RN...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Arboviruses represent major challenges to public health, particularly in tropical, and subtropical regions, and a substantial risk to other parts of the world as respective vectors extend their habitats. In recent years, two viruses transmitted by Aedes mosquitoes, Chikungunya and Zika virus, have gathered increased interest. After decades of regionally constrained outbreaks, both viruses have recently caused explosive outbreaks on an unprecedented scale, causing immense suffering and massive economic burdens in affected regions. Chikungunya virus causes an acute febrile illness that often transitions into a chronic manife...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Chikungunya virus (CHIKV) is the causative pathogen of chikungunya fever, a mosquito-borne viral disease causing highly debilitating arthralgia that can persist for months and progress to chronic arthritis. Our previous studies have identified the CHIKV live-attenuated vaccine candidate CHIKV-NoLS. Like most live-attenuated vaccines, attenuated replication of CHIKV-NoLS has the potential to limit scalable production. To overcome production limits, as well as other drawbacks of live-attenuated vaccines, we developed an in vivo liposome RNA delivery system to deliver the self-replicating RNA genome of CHIKV-NoLS directly int...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Bacteriophage Qβ virus-like particles displaying Chikungunya virus B-cell epitopes elicit high-titer E2 protein antibodies but fail to neutralize a Thailand strain of Chikungunya virus. Vaccine. 2020 Feb 07;: Authors: Basu R, Zhai L, Rosso B, Tumban E Abstract Chikungunya virus (CHIKV) is a mosquito-borne virus associated with arthritis and musculoskeletal pains. More than 2.9 million people worldwide have been infected with the virus within the last 1.5 decades; currently, there are no approved vaccines to protect against CHIKV infection. To assess the potential of using CHIKV peptides as vaccin...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
alérie Choumet Arboviruses like chikungunya and Ross River (RRV) are responsible for massive outbreaks of viral polyarthritis. There is no effective treatment or vaccine available against these viruses that induce prolonged and disabling arthritis. To explore the physiopathological mechanisms of alphaviral arthritis, we engineered a recombinant RRV expressing a NanoLuc reporter (RRV-NLuc), which exhibited high stability, near native replication kinetics and allowed real time monitoring of viral spread in an albino mouse strain. During the acute phase of the disease, we observed a high bioluminescent signal refle...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Tapas Kumar Nayak1†, Prabhudutta Mamidi2, Subhransu Sekhar Sahoo1, P. Sanjai Kumar1, Chandan Mahish1, Sanchari Chatterjee2, Bharat Bhusan Subudhi3, Soma Chattopadhyay2 and Subhasis Chattopadhyay1* 1School of Biological Sciences, National Institute of Science Education and Research, HBNI, Bhubaneswar, India2Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India3School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar, India Chikungunya virus (CHIKV), a mosquito-borne Alphavirus, is endemic in different parts of the globe. The host macrophages are identified as the maj...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusions Apart West Nile virus (78), thousands of congenital microcephaly cases, fetal brain tissue damage and neurological syndromes have been associated with ZIKV infection. Unfortunately, the epidemics of this mosquito born, and a relative stable virus is on a rise. Although congenital microcephaly is a rare disorder however, due to lack of standardized diagnostic test facilities, the incidence in the geographically widespread ZIKV epidemic regions is higher. Animals studies showed that ZIKV is a neurotropic virus. It directly targets the developing embryonic brain cells by inducing apoptosis, cell-cycle arrest, and...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
Purpose: We have developed a live recombinant measles vector-based vaccine to prevent Chikungunya disease (MV-CHIK). Previously, the safety and immunogenicity was demonstrated in a Phase 1 clinical trial (EudraCT: 2013-001084-23). Here, we will present the final data of a Phase 2 clinical trial in 263 healthy, volunteer adults (NCT02861586). Chikungunya is a rapidly spreading viral disease affecting significant parts of the Americas, India and South East Asia. In the majority of patients, the febrile acute disease turns into a chronic disease causing severe, debilitating arthritis that can last years post-infection.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 21.080 Source Type: research
Authors: McCarthy MK, Davenport BJJ, Morrison TE Abstract Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that has caused both small- and large-scale epidemics of incapacitating musculoskeletal disease across the globe. A substantial proportion of infected individuals experience debilitating arthralgia and/or arthritis that can persist in relapsing or continuous forms for months to years, an occurrence that appears independent of viral strain and outbreak location. Due to the lack of CHIKV-specific vaccine or therapeutics, treatment of chronic CHIKV disease is limited to supportive care. Although the...
Source: Current Topics in Microbiology and Immunology - Category: Microbiology Tags: Curr Top Microbiol Immunol Source Type: research
In conclusion, the ability of silymarin to inhibit MAYV replication and attenuate MAYV-induce oxidative stress warrants further investigation of this compound as a novel therapeutic approach to Mayaro fever disease.
Source: Antiviral Therapy - Category: Virology Source Type: research
More News: Arthritis | Biology | Chikungunya | Rheumatology | Vaccines | Washington University