Contemporary positional proteomics strategies to study protein processing.

Contemporary positional proteomics strategies to study protein processing.
Curr Opin Chem Biol. 2013 Jan 3;
Authors: Plasman K, Van Damme P, Gevaert K
Abstract
Positional proteomics refers to mass spectrometry (MS)-driven proteomics technologies by which peptides carrying the amino or carboxyl terminus of proteins are enriched from whole proteomes, and identified by means of MS. Proteases irreversibly modify their substrates by hydrolyzing peptide bonds and have thus profound effects on the biological processes steered by their substrates. Since proteases create novel termini in their substrates, positional proteomics is ideally suited for protease degradomics studies. We here review recent developments in the field of positional proteomics applied to protease research.
PMID: 23291282 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - January 3, 2013 Category: Biochemistry Authors: Plasman K, Van Damme P, Gevaert K Tags: Curr Opin Chem Biol Source Type: research