An investigation for recovery of polyhydroxyalkanoates (PHA) from Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19 isolated from plastic waste landfill.

An investigation for recovery of polyhydroxyalkanoates (PHA) from Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19 isolated from plastic waste landfill. Int J Biol Macromol. 2019 May 23;: Authors: Mohammed S, Panda AN, Ray L Abstract Bio-plastic synthesis from renewable and cheap agro-based materials is a sustainable solution for replacing conventionally produced plastic with environmental contamination. The current study was aimed at screening and characterization of Polyhydroxyalkanoates (PHA) producing bacterial isolates, evaluation of their potential and recovery of PHA using the isolates. The PHA compounds were characterized using FT-IR. Based on 16SrRNA sequence analyses the isolates were designated as Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19. The isolates were gram-positive, rod-shaped, endospore former, and citrate test positive. Intracellular PHA granules were observed when these isolates were stained with Sudan black B (SBB) and Nile blue A (NBA) preliminary and specific staining dyes, respectively. Effect of pH, temperature and carbon sources on the PHA production by the isolates BPPI-14 and BPPI-19 was studied. Maximum PHA production was recorded for Glucose (49.46 ± 2.79%) by Bacillus sp. BPPI-14 and followed by molasses (45.86 ± 2.17%) by Bacillus sp. BPPI-19, respectively at 37 °C and pH 7. The obtained PHA polymers were confirmed by preparation of plastic films for both the isolates. Fourier transform...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research