Detection of the Sickle Hemoglobin Allele using a Surface Plasmon Resonance Based Biosensor

Publication date: Available online 24 May 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Giulia Breveglieri, Elisabetta D’Aversa, Lucia Carmela Cosenza, Effrossyni Boutou, Angeliki Balassopoulou, Ersi Voskaridou, Roberto Gambari, Monica BorgattiAbstractSickle Cell Anemia (SCA) is a monogenic hereditary disease caused by a single point mutation (βS) in the β globin gene resulting in an abnormal hemoglobin (HbS) that can polymerize within the erythrocytes, inducing their characteristic sickle shape. This causes hemolytic anemia and occlusive vessels for the most severe clinical status. Molecular analysis is crucial for fast and precise diagnosis of different forms of SCA, and, on the basis of underlying genotype, for supporting the most appropriate treatment options. In this context, we describe a simple and reproducible protocol for the molecular identification of the βS mutation based on surface plasmon resonance (SPR) using the BiacoreTM X100 affinity biosensor. This technology has already demonstrated its diagnostic suitability for the identification of point mutations responsible for genetic diseases such as cystic fibrosis and β thalassemia, using a protocol based on immobilization of PCR products on the sensor chip. On the contrary, in this work we applied a SPR strategy based on an innovative interaction format, recently developed in our group also for β thalassemia mutations. In particular, we correctly detected the βS mutation responsible for SCA, bot...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research