N-Alkylated Chitosan/Graphene Oxide Porous Sponge for Rapid and Effective Hemostasis in Emergency Situations

Publication date: Available online 9 May 2019Source: Carbohydrate PolymersAuthor(s): Ying Zhang, Jing Guan, Jimin Wu, Sheng Ding, Jian Yang, Jianhua Zhang, Anjie Dong, Liandong DengAbstractN-alkylated chitosan (AC) sponges and graphene oxide (GO) sponges are promising candidates for emergency hemostat. However, AC sponges have weak mechanical strength and GO sponges may induce toxicity. To overcome these problems, a series of AC/GO composite spongs (ACGS) were prepared with various ratios (GO/AC, 0%, 5%, 10%, and 20%) using a dilute solution freeze phase separation and drying process. The sponges exhibit excellent absorption capacity, mechanical stability, and biocompatibility. In serial in vitro clotting tests, the higher the ratio of GO, the better the coagulation efficiency. ACGS with 20% ratio of GO (ACGS20) has shorter hemostatic time than Celox in a rabbit femoral injury test. Moreover, ACGS20 can accelerate erythrocyte and platelet adhesion. CD62p and intracellular Ca2+ measurements show that ACGS20 can promote the release of intracellular Ca2+ and stimulate platelet activation. These results suggest that ACGS20 is a good candidate composition for a safe and efficacious hemostatic dressing.Graphical abstract
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research