Multi-trait analysis of domestication genes in Cicer arietinum – Cicer reticulatum hybrids with a multidimensional approach: modeling wide crosses for crop improvement

Publication date: Available online 25 April 2019Source: Plant ScienceAuthor(s): Min-Gyoung Shin, Sergey V. Bulyntsev, Peter L. Chang, Lijalem Balcha Korbu, Noelia Carrasquila-Garcia, Margarita A. Vishnyakova, Maria G. Samsonova, Douglas R. Cook, Sergey V. NuzhdinAbstractDomestication and subsequent breeding have eroded genetic diversity in the modern chickpea crop by ˜100-fold. Corresponding reductions to trait variation create the need, and an opportunity, to identify and harness the genetic capacity of wild species for crop improvement. Here we analyze trait segregation in a series of wild x cultivated hybrid populations to delineate the genetic underpinnings of domestication traits. Two species of wild chickpea, C. reticulatum and C. echinospermum, were crossed with the elite, early flowering C. arietinum cultivar ICCV96029. KASP genotyping of F2 parents with an FT-linked molecular marker enabled selection of 284 F3 families with reduced phenological variation: 255 F3 families of C. arietinum x reticulatum (AR) derived from 17 diverse wild parents and 29 F3 families of C. arietinum x echinospermum (AE) from 3 wild parents. The combined 284 lineages were genotyped using a genotyping-by-sequencing strategy and phenotyped for agronomic traits. 50 QTLs in 11 traits were detected from AR and 35 QTLs in 10 traits from the combined data. Using hierarchical clustering to assign traits to six correlated groups and mixed model based multi-trait mapping, four pleiotropic loci were i...
Source: Plant Science - Category: Biology Source Type: research