Phylogeography and Epidemiology of Brucella suis biovar 2 in Wildlife and Domestic Swine

Publication date: Available online 22 April 2019Source: Veterinary MicrobiologyAuthor(s): Pilar María Muñoz, Virginie Mick, Lorena Sacchini, Anna Janowicz, María Jesús de Miguel, Moulay-Ali Cherfa, Celia Rodriguez Nevado, Guillaume Girault, Sara Andrés-Barranco, Maryne Jay, Elisabetta Di Giannatale, Katiuscia Zilli, Massimo Ancora, Alessandro Dondo, Simona Zoppi, María Cruz Arnal, Manuela Tittarelli, Fabrizio De Massis, Bruno Garin-Bastuji, José María BlascoAbstractSwine brucellosis due to Brucella suis biovar 2 (bv2) is enzootic in wild boar and hare in continental Europe and may cause major economic losses to the pig industry, mainly in free-ranged pig farms. The high nucleotide identity found among the B. suis biovar 2 isolates has long hindered the full understanding of the epidemiology and the phylogeography of the disease. Here, we used multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) and whole-genome analysis to identify single-nucleotide polymorphisms (SNPs) in order to gain insights from the largest B. suis bv2 dataset analyzed so far composed of domestic pigs and wildlife isolates collected throughout Europe since the 1970s. We found four major clades with a specific phylogeographic pattern. The Iberian clade contains isolates exclusively from the Iberian Peninsula. The Central European clade includes most isolates from France, Northern Italy, Switzerland and an important proportion of those of Northern Spain. The Eastern European clade cluste...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research