398 Neutrophil extracellular trap-dependent Th17 polarization of memory T-cells requires monocyte-T-cell contact and stimulates cytokine gene expression in an Act1D10N-dependent manner

We previously reported that neutrophil extracellular traps (NETs) increase the percentage of human Th17 cells in 7-day PBMC cultures, dependent upon CD3/CD28 stimulation and the presence of monocytes. Here we further characterize this response and its dependence upon the D10N variant of Act1 encoded by TRAF3IP2, which is genetically implicated in psoriasis and psoriatic arthritis. We fractionated PBMC into monocyte- and T-cell-enriched populations, then recombined them with or without separation by a Transwell filter.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research