Laser technology in the ART laboratory: a narrative review

Publication date: May 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 5Author(s): Lien M. Davidson, Yaqiong Liu, Tracey Griffiths, Celine Jones, Kevin CowardAbstractTo improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that the best quality gametes and embryos can be identified and manipulated, thus improving clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as assisted hatching, embryo biopsy, intracytoplasmic sperm injection cryopreservation and sperm immobilization/selection. This review examines the current status of cutting-edge laser-assisted reproductive technologies, investigates experimental techniques that are increasingly being applied clinically. It highlights the benefits of lasers as a powerful technology at the forefront of both diagnostic and therapeutic treatments for general subfertility and male-factor infertility. However, it is important to note that although lasers are becoming increasingly commonplace in ART units, there is comparatively little information in the existing literature pertaining to the potential negative effects that laser application might have on the developing human embryo, thus creating the need for further investigative research.
Source: Reproductive BioMedicine Online - Category: Reproduction Medicine Source Type: research

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ConclusionOur results suggested HBV infection did not appear to be detrimental to the outcome of IVF/ICSI treatment.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
ConclusionsThis sequence variation of DEFB126 may impair male reproductive function and can be related to male infertility. Consequently, the fertility rate is significantly less in del/del males than wt/wt males. Interestingly, males with the del/del genotype have normal spermogeram however, their sperm is functionally impaired, which can affect IUI treatment outcome.
Source: Reproductive BioMedicine Online - Category: Reproduction Medicine Source Type: research
Purpose of review The impact of intracytoplasmic sperm injection (ICSI), on the reproductive health of the offspring is largely unknown. Here we provide a comprehensive overview of the endocrine and reproductive profile in boys and young male adults born after ICSI using ejaculated spermatozoa alleviating male factor infertility in their parents. Recent findings Levels of testosterone, anti-Müllerian hormone and inhibin B were found comparable in prepubertal and pubertal boys conceived by ICSI when compared with levels in boys conceived spontaneously. Also, at young adulthood, mean levels of reproductive hormones...
Source: Current Opinion in Obstetrics and Gynecology - Category: OBGYN Tags: FERTILITY, IVF AND REPRODUCTIVE GENETICS: Edited by Emre Seli and Juan A. GarcĂ­a Velasco Source Type: research
Condition:   Infertility Intervention:   Drug: Prontogest 400 mg vaginal pessaries Sponsors:   Ain Shams Maternity Hospital;   Ahmed Mahmoud Abdel-Rahim Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
(Abstracted from Fertil Steril 2019;111(1):86–96) Initial phases of embryonic development have a high requirement for ATP, and evidence shows that oocytes with high ATP levels enhance implantation of the human embryo. Decreased bioenergetic capacity in oocytes has been found to be a primary contributor to declining embryo quality with advancing maternal age or in women with premature ovarian aging.
Source: Obstetrical and Gynecological Survey - Category: OBGYN Tags: GYNECOLOGY: INFERTILITY/REPRODUCTIVE TECHNOLOGIES Source Type: research
At present, the precise role of human menopausal gonadotropin (HMG) and recombinant luteinizing hormone (rLH) supplementation at an early time of follicular phase on in vitro fertilization (IVF)/intra cytoplasmatic sperm injection (ICSI) outcomes remains uncertain. Here infertile women of normal ovarian function undergoing their first cycle of IVF/ICSI were studied and were randomly allocated into 3 groups. Group 1, ovarian stimulation with 150IU recombinant follicle-stimulating hormone (FSH) alone. Group 2, patients received 75IU rFSH and 75IU HMG. Group 3 patients were given 150IU rFSH and 75IU rLH. There were no signi...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
Frank J. Broekmans* University Medical Center Utrecht, Utrecht, Netherlands The art of ovarian stimulation for IVF/ICSI treatment using exogenous FSH should be balanced against the relative contribution of other steps of the ART process such as the IVF-lab-phase and the Embryo-Transfer. The aim of ovarian stimulation is to obtain a certain number of oocytes, that will enable the best probability of achieving a live birth. It has been suggested that more oocytes will create a better prospect for pregnancy, but studies on the question whether the retrieval of a few oocytes less or more will make the difference are ...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
This paper discusses the variety of effective sperm selection techniques that have been developed for use in assisted reproductive technologies. Available methods for isolating the competent sperm in an ejaculate are outlined, as well as techniques for selecting single sperm for use in intracytoplasmic sperm injection procedures. Case-specific methods for selecting the most competent sperm are discussed, with reference to the potential causes of male factor infertility and guidance for the embryologist based on the issues present for each couple seeking treatment.
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: Views and reviews Source Type: research
Infertility due to obstructive azoospermia may be treated effectively by surgical reconstruction or by retrieval of sperm from the epididymis or testis, followed by in  vitro fertilization with intracytoplasmic sperm injection. This replaces the ASRM documents titled “Sperm retrieval for obstructive azoospermia” and “The management of infertility due to obstructive azoospermia,” last published in 2008.
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: ASRM pages Source Type: research
Conclusion The periconceptional environment and lifestyle factors modify sperm epigenome. This alteration might be maintained in the zygote and throughout development, thereby leading to the inheritance of newly acquired pathologies. The role of sperm miRNA, not only as innovative markers of fertility issues but also as vectors involved in the inheritance of paternal diseases, appears to be crucial. Overweight and obesity seem to alter sperm miRNA profile, thereby leading to transmission of different miRNA profiles in zygote, with consequences on embryo development. In long term, metabolic disorders have been described in...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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