Effects of different levels of soluble PD-L1 protein on the growth of Lewis lung cancer transplanted tumor.

Effects of different levels of soluble PD-L1 protein on the growth of Lewis lung cancer transplanted tumor. J Biol Regul Homeost Agents. 2019 Mar-Apr;33(2):537-542 Authors: Yang KN, Han W, Qin YJ, Chen LN Abstract To investigate the effects of different doses of soluble PD-L1 (soluble form of Programmed death ligand 1, sPD-L1) protein on Lewis lung cancer cells, flow cytometry was used to detect the expression of PD-L1 (Programmed death ligand 1) on the surface of Lewis lung cancer cell lines and the expression of PD-1 on the surface of T lymphocytes in peripheral blood and spleen cells of C57BL/6 mice. A Lewis lung cancer animal model of C57BL/6 mice was established by transplanting Lewis lung cancer cells subcutaneously. The sPD-L1 protein was injected into the abdominal cavity of the mouse (sPD-L1 Ig) (working dose: 2.5, 5, 10 μg per mouse), while the sPD-L1 control protein was injected as a control. The growth of Lewis lung cancer xenografts was observed. On the 18th day after tumor cell inoculation, T lymphocyte subsets in mouse spleen were determined by flow cytometry. The PD-1 molecules on the surface of Lewis lung cancer cell line, C57BL/6 mouse spleen T lymphocytes and peripheral blood T lymphocytes were positively expressed. Compared with the control group, the volume of the transplanted tumor of Lewis lung cancer in C57BL/6 mice was larger with 10 μg sPD-L1 I g injection (P less than 0.05), and no significant difference ...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research