Activatable NIRF/MRI dual imaging probe using bio-inspired coating of glycol chitosan on superparamagnetic iron oxide nanoparticles

Publication date: Available online 12 April 2019Source: Journal of Industrial and Engineering ChemistryAuthor(s): In Jae Chung, Seong Ik Jeon, Eui-Joon Cha, Youngro Byun, Ick Chan Kwon, Young Il Kim, Kwangmeyung Kim, Cheol-Hee AhnAbstractMRI and NIRF multimodal imaging probe was fabricated and investigated. Glycol chitosan coated SPION was decorated by MMP-2 substrate conjugating BHQ-3 and Cy5.5 dye at each end (BCMG-SPION). NIRF signals were recovered in the presence of MMP-2 at a sub-nanomolar level. BCMG-SPION produced the maximum NIRF intensity at 48 h post injection in vivo. Evaluation of the BCMG-SPION in the tumor tissue by MRI showed that relative contrast enhancement was 34 % higher than normal muscles. NIRF images of excised organs showed the highest NIRF signal in tumor and the intensity was at least 8 times higher than other organs.Graphical abstractMultimodal Molecular Imaging: SPION was coated by biocompatible glycol chitosan conjugating activatable NIRF imaging molecules via bio-inspired adhesion and its potential as a dual MRI/NIRF imaging probe for cancer imaging was demonstrated. Tumor was selectively imaged by dual modalities based on the nanoparticular nature and pH-tunable coating layer.
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research