Development and annotation of species-specific microsatellite markers from transcriptome sequencing for a higher group termite, Globitermes sulphureus Haviland (Blattodea: Termitidae)

In this study, samples of G. sulphureus were collected from 10 grounded populations and another 10 native populations throughout the Northern part of Malaysia. 30 novel polymorphic microsatellite markers were developed and characterized from transcriptome dataset of urban-originating G. sulphureus enriched for di-, tri- and tetranucleotide repeat motifs. Thirty primer pairs were successfully amplified DNA fragments from 600 isolates of G. sulphureus, revealing an overall of 180 alleles. The number of alleles per locus identified ranged from 3 to 9. Among sequences containing microsatellites, dinucleotides repeats were the most abundant (44.57%), followed by trinucleotides repeats (41.99%), tetranucleotides repeats (11.18%), pentanucleotides repeats (1.84%), and hexanucleotides repeats (0.42%). This is the first report of microsatellite polymorphisms in G. sulphureus. The microsatellite primers developed in this current work may facilitate in population genetic study of this termite species.
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research