Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

In this study, we used a highly sensitive proteomics approach combining affinity enrichment with high ‐resolution liquid chromatography coupled with tandem mass spectrometry to examine Ksuc inP gingivalis. In total, 345 Ksuc sites in 233 proteins were identified and determined to be involved in a variety of cellular processes. In the region surrounding Ksuc sites, lysine residues were drastically overrepresented and sequence motifs with succinyl ‐lysine flanked by a lysine at the +3 or +6 positions appear to be unique to this pathogen. Additionally, our results suggest a crosstalk between Ksuc and glycosylation, but the overlap between Ksuc and acetylation inP gingivalis is quite different from that observed in other organisms. Notably, Ksuc was observed in proteins associated with established virulence factors, including gingipains, fimbriae, RagB, and PorR. Moreover, products of the factors necessary forP gingivalis in vitro survival (18.5%) were found to be succinylated at lysine sites and the same was observed in products of fitness factors forP gingivalis survival in both abscess and epithelial cell colonization environments (12%). Collectively, these results suggest that Ksuc may be a new mechanism in modulating the virulence, adaptation, and fitness ofP gingivalis.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research