Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation.

Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation.
Talanta. 2019 Jun 01;198:424-431
Authors: Tortajada-Genaro LA, Yamanaka ES, Maquieira Á
Abstract
Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation, was developed and integrated into user-friendly optical devices: e.g., USB digital microscope, flatbed scanner, smartphone and DVD drive. In order to adequately identify a single base change, loop-mediated isothermal amplification (LAMP) was employed, with high yields (8 orders) within 45 min. Subsequently, products were directly hybridised to the allele-specific probes attached to plastic chips in an array format. After colorimetric staining, four consumer electronic techniques were compared. Sensitive precise measurements were taken (high signal-to-noise ratios, 10-μm image resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs. The analytical performances of the integrated platform (assay and reader) in t...

Source: Talanta - March 18, 2019 Category: Chemistry Authors: Tortajada-Genaro LA, Yamanaka ES, Maquieira Á Tags: Talanta Source Type: research