Biosynthesis of selenium nanoparticles mediated by fungus Mariannaea sp. HJ and their characterization

Publication date: Available online 16 March 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Henglin Zhang, Hao Zhou, Jiandong Bai, Yan Li, Jing Yang, Qiao Ma, Qu YuanyuanAbstractThe biosynthesis of selenium nanoparticles (SeNPs) by Mariannaea sp. HJ was reported for the first time. Different cultural conditions, including SeO2 concentrations and pH were studied, 2 mM SeO2 and pH 10 were favorable for the removal of Se(IV), and extracellular SeNPs were observed at alkaline pH values. UV-vis spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize SeNPs. The results showed that removal of Se(IV) ions occurred during the late exponential phase of strain HJ. TEM and SEM images revealed that spherical nanoparticles were deposited on the cell wall and in the cytoplasmic region of strain HJ with a uniform size distribution. XRD pattern suggested the standard hexagonal phase of selenium crystals. FTIR, XPS and selective passivation experiments implicated that lipid and amide groups were involved in the formation process and Se(-I) or Se(-II) species were demonstrated to be functioned in this process. Based on the SDS-PAGE analysis, the up-regulated proteins of MW ∼91.6 and ∼37.4-57.2 kDa might promote SeNP formation, and a number of prot...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research